Name: Anti-GRAP2 Antibody (CAB8767)
Brand: Cell Biology Antibodies 12
SKU: CAB8767
Availability: OutOfStock

Antibody Name:	Anti-GRAP2 Antibody
Antibody SKU:	CAB8767
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human GRAP2 (NP_004801.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human GRAP2 (NP_004801.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEAV AKFD FTAS GEDE LSFH TGDV LKIL SNQE EWFK AELG SQEG YVPK NFID IQFP KWFH EGLS RHQA ENLL MGKE VGFF IIRA SQSS PGDF SISV RHED DVQH FKVM RDNK GNYF LWTE KFPS LNKL VDYY RTNS ISRQ KQIF LRDR TRED QGHR GNSL DRRS QGGP HLSG AVGE EIRP SMNR KLSD HPPT LPLQ QHQH QPQP PQYA PAPQ QLQQ PPQQ RYLQ HHHF HQER RGGS LDIN DGHC GTGL GSEM NAAL MHRR HTDP VQLQ AAGR VRWA RALY DFEA LEDD ELGF HSGE VVEV LDSS NPSW WTGR LHNK LGLF PANY VAPM TR
Gene ID:	9402
Uniprot:	O75791
Cellular Location:	Cytoplasm, Endosome, Nucleus
Calculated MW:	24kDa/37kDa
Observed MW:	38kDa

Synonyms:

GRAP2, GADS, GRAP-2, GRB2L, GRBLG, GRID, GRPL, GrbX, Grf40, Mona, P38

Background:

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	GADS: Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. Belongs to the GRB2/sem-5/DRK family.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: cytoplasm; cytosol; endosome; nucleoplasm; nucleus Molecular Function:protein binding; SH3/SH2 adaptor activity Biological Process: cell-cell signaling; innate immune response; positive regulation of signal transduction; Ras protein signal transduction; T cell costimulation; T cell receptor signaling pathway
NCBI Summary:	This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
UniProt Code:	O75791
NCBI GenInfo Identifier:	6685489
NCBI Gene ID:	9402
NCBI Accession:	O75791.1
UniProt Secondary Accession:	O75791,O43726, Q9NRB7, B7Z8I3,
UniProt Related Accession:	O75791
Molecular Weight:	24,382 Da
NCBI Full Name:	GRB2-related adapter protein 2
NCBI Synonym Full Names:	GRB2-related adaptor protein 2
NCBI Official Symbol:	GRAP2
NCBI Official Synonym Symbols:	P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
NCBI Protein Information:	GRB2-related adapter protein 2
UniProt Protein Name:	GRB2-related adapter protein 2
UniProt Synonym Protein Names:	Adapter protein GRID; GRB-2-like protein; GRB2L; GRBLG; GRBX; Grf40 adapter protein; Grf-40; Growth factor receptor-binding protein; Hematopoietic cell-associated adapter protein GrpL; P38; Protein GADS; SH3-SH2-SH3 adapter Mona
Protein Family:	GRB2-related adapter protein
UniProt Gene Name:	GRAP2
UniProt Entry Name:	GRAP2_HUMAN

Anti-GRAP2 Polyclonal Antibody (CAB8767)

Western blot analysis of extracts of Jurkat cells, using GRAP2 antibody (CAB8767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.

Antibodies

GRAP2 Antibody (PACO06501)

GRAP2 Antibody (PACO09581)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)