Anti-Granulin Antibody (CAB20806)

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SKU:
CAB20806
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Goat
Isotype:
IgG

Description

Antibody Name:Anti-Granulin Antibody (CAB20806)
Antibody SKU:CAB20806
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human Granulin (NP_002078.1).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-431, HepG2, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human Granulin (NP_002078.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CPDG QFCP VACC LDPG GASY SCCR PLLD KWPT TLSR HLGG PCQV DAHC SAGH SCIF TVSG TSSC CPFP EAVA CGDG HHCC PRGF HCSA DGRS CFQR SGNN SVGA IQCP DSQF ECPD FSTC CVMV DGSW GCCP MPQA SCCE DRVH CCPH GAFC DLVH TRCI TPTG THPL AKKL PAQR TNRA V
Cellular Location:Secreted
Calculated MW:44kDa/46kDa/63kDa
Observed MW:65-75KDa
Synonyms:GRN, CLN11, GEP, GP88, PCDGF, PEPI, PGRN, granulins
Background:Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
Western blot analysis of extracts of various cell lines, using Granulin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Western blot analysis of extracts of various cell lines, using Granulin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
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