Antibody Name:	Anti-GPX4 Antibody
Antibody SKU:	CAB13309
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-197 of human GPX4 (NP_002076.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-197 of human GPX4 (NP_002076.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ASRD DWRC ARSM HEFS AKDI DGHM VNLD KYRG FVCI VTNV ASQU GKTE VNYT QLVD LHAR YAEC GLRI LAFP CNQF GKQE PGSN EEIK EFAA GYNV KFDM FSKI CVNG DDAH PLWK WMKI QPKG KGIL GNAI KWNF TKFL IDKN GCVV KRYG PMEE PLVI EKDL PHYF
Gene ID:	2879
Uniprot:	P36969
Cellular Location:	Cytoplasm, Mitochondrion
Calculated MW:	19kDa/22kDa
Observed MW:	17KDa

Synonyms:

GPX4, GPx-4, GSHPx-4, MCSP, PHGPx, SMDS, snGPx, snPHGPx

Background:

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	GPX4: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:	Protein type:Other Amino Acids Metabolism - glutathione; Nuclear envelope; Lipid Metabolism - arachidonic acid; EC 1.11.1.12; Oxidoreductase; Mitochondrial Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: mitochondrion; nucleus; cytosol Molecular Function:selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity Biological Process: glutathione metabolic process; lipoxygenase pathway; hydrogen peroxide catabolic process; multicellular organismal development; phospholipid metabolic process; regulation of inflammatory response; arachidonic acid metabolic process; response to oxidative stress; response to estradiol stimulus; aging
NCBI Summary:	This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:	P36969
NCBI GenInfo Identifier:	172045844
NCBI Gene ID:	2879
NCBI Accession:	P36969.3
UniProt Secondary Accession:	P36969,O43381, Q6PJ59, Q9UPK2,
UniProt Related Accession:	P36969
Molecular Weight:	Observed MW: 22kDaCalculated MW: 19kDa/22kDa
NCBI Full Name:	Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
NCBI Synonym Full Names:	glutathione peroxidase 4
NCBI Official Symbol:	GPX4
NCBI Official Synonym Symbols:	MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
NCBI Protein Information:	phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase
UniProt Protein Name:	Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
UniProt Synonym Protein Names:	Glutathione peroxidase 4; GPx-4; GSHPx-4
Protein Family:	Probable glutathione peroxidase
UniProt Gene Name:	GPX4
UniProt Entry Name:	GPX4_HUMAN

	Western blot analysis of extracts of various cell lines, using GPX4 antibody (CAB13309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat testis using GPX4 Rabbit pAb (CAB13309) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using GPX4 Rabbit pAb (CAB13309) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using GPX4 Rabbit pAb (CAB13309) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using GPX4 Rabbit pAb (CAB13309) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.