Antibody Name:	Anti-GPX2 Antibody
Antibody SKU:	CAB15999
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-180 of human GPX2 (NP_002074.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HT-29, Mouse stomach, Rat small intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-180 of human GPX2 (NP_002074.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TLVQ KCEV NGQN EHPV FAYL KDKL PYPY DDPF SLMT DPKL IIWS PVRR SDVA WNFE KFLI GPEG EPFR RYSR TFPT INIE P
Gene ID:	2877
Uniprot:	P18283
Cellular Location:
Calculated MW:	21kDa
Observed MW:	21kDa

Synonyms:

GPX2, GI-GPx, GPRP, GPRP-2, GPx-2, GPx-GI, GSHPX-GI, GSHPx-2

Background:

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	GPX2: Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors. Belongs to the glutathione peroxidase family.
UniProt Protein Details:	Protein type:Other Amino Acids Metabolism - glutathione; EC 1.11.1.9; Oxidoreductase; Lipid Metabolism - arachidonic acid Chromosomal Location of Human Ortholog: 14q24.1 Cellular Component: cytoplasm; cytosol Molecular Function:electron carrier activity; glutathione peroxidase activity Biological Process: gene expression; response to reactive oxygen species; transcription initiation from RNA polymerase II promoter
NCBI Summary:	This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. The protein encoded by this locus contains a selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]
UniProt Code:	P18283
NCBI GenInfo Identifier:	32967607
NCBI Gene ID:	2877
NCBI Accession:	NP_002074.2
UniProt Secondary Accession:	P18283,Q6PJ52, Q8WWI7, Q9NRP9,
UniProt Related Accession:	P18283
Molecular Weight:	21,954 Da
NCBI Full Name:	glutathione peroxidase 2
NCBI Synonym Full Names:	glutathione peroxidase 2
NCBI Official Symbol:	GPX2
NCBI Official Synonym Symbols:	GPRP; GPx-2; GI-GPx; GPRP-2; GPx-GI; GSHPx-2; GSHPX-GI
NCBI Protein Information:	glutathione peroxidase 2
UniProt Protein Name:	Glutathione peroxidase 2
UniProt Synonym Protein Names:	Gastrointestinal glutathione peroxidase; Glutathione peroxidase-gastrointestinal; GPx-GI; GSHPx-GI; Glutathione peroxidase-related protein 2; GPRP-2
Protein Family:	Glutathione peroxidase
UniProt Gene Name:	GPX2
UniProt Entry Name:	GPX2_HUMAN

	Western blot analysis of extracts of various cell lines, using GPX2 antibody (CAB15999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using GPX2 antibody (CAB15999) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat stomach using GPX2 antibody (CAB15999) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using GPX2 antibody (CAB15999) at dilution of 1:100 (40x lens).