Antibody Name:	Anti-GPT Antibody
Antibody SKU:	CAB2814
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human GPT (NP_005300.1).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse lung, Mouse intestine, Rat liver, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human GPT (NP_005300.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASS TGDR SQAV RHGL RAKV LTLD GMNP RVRR VEYA VRGP IVQR ALEL EQEL RQGV KKPF TEVI RANI GDAQ AMGQ RPIT FLRQ VLAL CVNP DLLS SPNF PDDA KKRA ERIL QACG GHSL GAYS VSSG IQLI REDV ARYI ERRD GGIP ADPN NVFL STGA SDAI VTVL KLLV AGEG HTRT GVLI PIPQ YP
Gene ID:	2875
Uniprot:	P24298
Cellular Location:	Cytoplasm
Calculated MW:	54kDa
Observed MW:	55kDa

Synonyms:

GPT, AAT1, ALT1, GPT1

Background:

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

UniProt Protein Function:	GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
UniProt Protein Details:	Protein type:EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: cytosol Molecular Function:alanine transaminase activity Biological Process: amino acid biosynthetic process
NCBI Summary:	This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
UniProt Code:	P24298
NCBI GenInfo Identifier:	46577683
NCBI Gene ID:	2875
NCBI Accession:	P24298.3
UniProt Secondary Accession:	P24298,P78398, Q93076, B0YJ18, D3DWM7,
UniProt Related Accession:	P24298
Molecular Weight:	54,637 Da
NCBI Full Name:	Alanine aminotransferase 1
NCBI Synonym Full Names:	glutamic--pyruvic transaminase
NCBI Official Symbol:	GPT
NCBI Official Synonym Symbols:	AAT1; ALT1; GPT1
NCBI Protein Information:	alanine aminotransferase 1
UniProt Protein Name:	Alanine aminotransferase 1
UniProt Synonym Protein Names:	Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
Protein Family:	Alanine aminotransferase
UniProt Gene Name:	GPT
UniProt Entry Name:	ALAT1_HUMAN

	Western blot analysis of extracts of various cell lines, using GPT antibody (CAB2814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using GPT antibody (CAB2814) at dilution of 1:100 (40x lens).