Cell Biology Antibodies 12

Anti-GPRC6A Antibody (CAB8525)

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SKU:
CAB8525
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GPRC6A Antibody
Antibody SKU:CAB8525
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human GPRC6A (NP_683766.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, Mouse liver, Mouse lung, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human GPRC6A (NP_683766.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IASD NWST ATKI TTIP NVKK IGKV VGFA FRRG NISS FHSF LQNL HLLP SDSH KLLH EYAM HLSA CAYV KDTD LSQC IFNH SQRT LAYK ANKA IERN FVMR NDFL WDYA EPGL IHSI QLAV FALG YAIR DLCQ ARDC QNPN AFQP WELL GVLK NVTF TDGW NSFH FDAH GDLN TGYD VVLW KEIN GHMT VTKM AEYD LQND VFII PDQE TKNE FRNL KQIQ SKCS KECS PGQM KKTT RSQH ICCY ECQN CPEN HYTN QTDM PHCL LCNN KTHW APVR STMC FEKE VEYL NWN
Gene ID:222545
Uniprot:Q5T6X5
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:84kDa/96kDa/104kDa
Observed MW:120kDa
Synonyms:GPRC6A, GPCR, bA86F4.3
Background:
UniProt Protein Function:GPRC6A: Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca(2+) and Mg(2+). Activated by extracellular calcium and osteocalin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinates nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalents ions and its responsiveness to anabolic steroids. Belongs to the G-protein coupled receptor 3 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; GPCR, family 3; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: plasma membrane

Biological Process: response to amino acid stimulus

NCBI Summary:Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).[supplied by OMIM, Nov 2010]
UniProt Code:Q5T6X5
NCBI GenInfo Identifier:74745292
NCBI Gene ID:222545
NCBI Accession:Q5T6X5.1
UniProt Secondary Accession:Q5T6X5,Q6JK43, Q6JK44, Q8NGU8, Q8NHZ9, Q8TDT6,
UniProt Related Accession:Q5T6X5
Molecular Weight:96,526 Da
NCBI Full Name:G-protein coupled receptor family C group 6 member A
NCBI Synonym Full Names:G protein-coupled receptor class C group 6 member A
NCBI Official Symbol:GPRC6A  
NCBI Official Synonym Symbols:GPCR; bA86F4.3  
NCBI Protein Information:G-protein coupled receptor family C group 6 member A
UniProt Protein Name:G-protein coupled receptor family C group 6 member A
UniProt Synonym Protein Names:G-protein coupled receptor GPCR33; hGPCR33
Protein Family:G-protein coupled receptor family
UniProt Gene Name:GPRC6A  
UniProt Entry Name:GPC6A_HUMAN
Anti-GPRC6A Polyclonal Antibody (CAB8525)Western blot analysis of extracts of various cell lines, using GPRC6A antibody (CAB8525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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