Cell Biology Antibodies 11

Anti-GPR68 Antibody (CAB7348)

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SKU:
CAB7348
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GPR68 Antibody
Antibody SKU:CAB7348
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 276-365 of human GPR68 (NP_003476.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, U-87MG, NIH/3T3, Jurkat, Mouse brain, Mouse spleen, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 276-365 of human GPR68 (NP_003476.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SFNC VADP VLYC FVSE TTHR DLAR LRGA CLAF LTCS RTGR AREA YPLG APEA SGKS GAQG EEPE LLTK LHPA FQTP NSPG SGGF PTGR LA
Gene ID:8111
Uniprot:Q15743
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:41kDa
Observed MW:48kDa
Synonyms:GPR68, AI2A6, GPR12A, OGR1
Background:The protein encoded by this gene is a G protein-coupled receptor for sphingosylphosphorylcholine. The encoded protein is a proton-sensing receptor, inactive at pH 7.8 but active at pH 6.8. Mutations in this gene are a cause of amelogenesis imperfecta.
UniProt Protein Function:GPR68: Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca(2+) mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Function also as a metastasis suppressor gene in prostate cancer. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:

Protein type:Tumor suppressor; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 14q31

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function:G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; inflammatory response; negative regulation of monocyte differentiation

UniProt Code:Q15743
NCBI GenInfo Identifier:3024266
NCBI Gene ID:8111
NCBI Accession:Q15743.1
UniProt Secondary Accession:Q15743,Q13334, Q4VBB4, Q6IX34,
UniProt Related Accession:Q15743
Molecular Weight:365
NCBI Full Name:Ovarian cancer G-protein coupled receptor 1
NCBI Synonym Full Names:G protein-coupled receptor 68
NCBI Official Symbol:GPR68  
NCBI Official Synonym Symbols:OGR1; GPR12A  
NCBI Protein Information:ovarian cancer G-protein coupled receptor 1; G-protein coupled receptor 68; sphingosylphosphorylcholine receptor; ovarian cancer G protein-coupled receptor, 1
UniProt Protein Name:Ovarian cancer G-protein coupled receptor 1
UniProt Synonym Protein Names:G-protein coupled receptor 68; GPR12A; Sphingosylphosphorylcholine receptor
Protein Family:Ovarian cancer G-protein coupled receptor
UniProt Gene Name:GPR68  
UniProt Entry Name:OGR1_HUMAN
Anti-GPR68 Antibody (CAB7348)Western blot analysis of extracts of various cell lines, using GPR68 antibody (CAB7348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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