Cell Biology Antibodies 4
Anti-GPNMB Antibody (CAB14270)
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- SKU:
- CAB14270
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GPNMB Antibody |
| Antibody SKU: | CAB14270 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-260 of human GPNMB (NP_001005340.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, K-562, U-87MG, Mouse brain, Mouse liver, Rat brain, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-260 of human GPNMB (NP_001005340.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AKRF HDVL GNER PSAY MREH NQLN GWSS DEND WNEK LYPV WKRG DMRW KNSW KGGR VQAV LTSD SPAL VGSN ITFA VNLI FPRC QKED ANGN IVYE KNCR NEAG LSAD PYVY NWTA WSED SDGE NGTG QSHH NVFP DGKP FPHH PGWR RWNF IYVF HTLG QYFQ KLGR CSVR VSVN TANV TLGP QLME VTVY RRHG RAYV PIAQ VKDV YVVT DQIP VFVT MFQK NDRN SSDE TFLK DLP |
| Gene ID: | 10457 |
| Uniprot: | Q14956 |
| Cellular Location: | Cell membrane, Melanosome, Single-pass type I membrane protein |
| Calculated MW: | 62kDa/63kDa |
| Observed MW: | 64kDa |
| Synonyms: | GPNMB, HGFIN, NMB |
| Background: | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | GPNMB: Could be a melanogenic enzyme. Belongs to the PMEL/NMB family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 7p15 Cellular Component: integral to plasma membrane; melanosome; integral to membrane Molecular Function:integrin binding; heparin binding Biological Process: osteoblast differentiation; negative regulation of cell proliferation; cell adhesion; bone mineralization |
| NCBI Summary: | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14956 |
| NCBI GenInfo Identifier: | 61252504 |
| NCBI Gene ID: | 10457 |
| NCBI Accession: | Q14956.2 |
| UniProt Secondary Accession: | Q14956,Q6UVX1, Q8N1A1, A4D155, |
| UniProt Related Accession: | Q14956 |
| Molecular Weight: | 62,643 Da |
| NCBI Full Name: | Transmembrane glycoprotein NMB |
| NCBI Synonym Full Names: | glycoprotein (transmembrane) nmb |
| NCBI Official Symbol: | GPNMB |
| NCBI Official Synonym Symbols: | NMB; HGFIN |
| NCBI Protein Information: | transmembrane glycoprotein NMB; osteoactivin; glycoprotein nmb-like protein; transmembrane glycoprotein HGFIN |
| UniProt Protein Name: | Transmembrane glycoprotein NMB |
| UniProt Synonym Protein Names: | Transmembrane glycoprotein HGFIN |
| Protein Family: | Transmembrane glycoprotein |
| UniProt Gene Name: | GPNMB |
| UniProt Entry Name: | GPNMB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GPNMB antibody (CAB14270) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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