Antibody Name:	Anti-GPLD1 Antibody
Antibody SKU:	CAB6612
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPLD1 (NP_001494.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse liver, Mouse brain, Rat liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPLD1 (NP_001494.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CGLS THVE IGHR ALEF LQLH NGRV NYRE LLLE HQDA YQAG IVFP DCFY PSIC KGGK FHDV SEST HWTP FLNA SVHY IREN YPLP WEKD TEKL VAFL FGIT SHMA ADVS WHSL GLEQ GFLR TMGA IDFH GSYS EAHS A
Gene ID:	2822
Uniprot:	P80108
Cellular Location:	Secreted
Calculated MW:	19kDa/92kDa
Observed MW:	92kDa

Synonyms:

GPLD1, GPIPLD, GPIPLDM, PIGPLD, PIGPLD1, PLD

Background:

Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.

UniProt Protein Function:	GPLD1: This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. Belongs to the GPLD1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Secreted; Secreted, signal peptide; Phospholipase; EC 3.1.4.50 Chromosomal Location of Human Ortholog: 6p22.1 Cellular Component: extracellular space; proteinaceous extracellular matrix; intracellular membrane-bound organelle; lysosomal membrane; cytoplasm; intracellular Molecular Function:glycosylphosphatidylinositol phospholipase D activity; sodium channel regulator activity; phospholipase D activity Biological Process: ossification; GPI anchor release; positive regulation of apoptosis; complement receptor mediated signaling pathway; positive regulation of membrane protein ectodomain proteolysis; phosphatidylcholine metabolic process; negative regulation of cell proliferation; cellular response to insulin stimulus; cell migration during sprouting angiogenesis; insulin receptor signaling pathway; response to glucose stimulus; positive regulation of secretion; chondrocyte differentiation; positive regulation of cytolysis
NCBI Summary:	Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]
UniProt Code:	P80108
NCBI GenInfo Identifier:	126302583
NCBI Gene ID:	2822
NCBI Accession:	P80108.3
UniProt Related Accession:	P80108
Molecular Weight:
NCBI Full Name:	Phosphatidylinositol-glycan-specific phospholipase D
NCBI Synonym Full Names:	glycosylphosphatidylinositol specific phospholipase D1
NCBI Official Symbol:	GPLD1
NCBI Official Synonym Symbols:	PLD; GPIPLD; PIGPLD; GPIPLDM; PIGPLD1
NCBI Protein Information:	phosphatidylinositol-glycan-specific phospholipase D
UniProt Protein Name:	Phosphatidylinositol-glycan-specific phospholipase D
UniProt Synonym Protein Names:	Glycoprotein phospholipase D; Glycosyl-phosphatidylinositol-specific phospholipase D; GPI-PLD; GPI-specific phospholipase D
UniProt Gene Name:	GPLD1
UniProt Entry Name:	PHLD_HUMAN

	Western blot analysis of extracts of various cell lines, using GPLD1 antibody (CAB6612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat heart using GPLD1 antibody (CAB6612) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using GPLD1 antibody (CAB6612) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using GPLD1 antibody (CAB6612) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using GPLD1 antibody (CAB6612). Blue: DAPI for nuclear staining.