Epigenetics & Nuclear Signaling Antibodies 1

Anti-GPKOW Antibody (CAB13214)

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SKU:
CAB13214
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-GPKOW Antibody
Antibody SKU:CAB13214
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 127-476 of human GPKOW (NP_056513.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 127-476 of human GPKOW (NP_056513.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TLAI PMIQ KGCT PSGE GADS EPRA ETVP EEAN YEAV PVEA YGLA MLRG MGWK PGEG IGRT FNQV VKPR VNSL RPKG LGLG ANLT EAQA LTPT GPSR MPRP DEEQ EKDK EDQP QGLV PGGA VVVL SGPH RGLY GKVE GLDP DNVR AMVR LAVG SRVV TVSE YYLR PVSQ QEFD KNTL DLRQ QNGT ASSR KTLW NQEL YIQQ DNSE RKRK HLPD RQDG PAAK SEKA APRS QHWL HRDL RVRF VDNM YKGG QYYN TKMI IEDV LSPD TCVC RTDE GRVL EGLR EDML ETLV PKAE GDRV MVVL GPQT GRVG HLLS RDRA RSRA LVQL PREN QVVE LHYD AICQ YMGP SDTD DD
Gene ID:27238
Uniprot:Q92917
Cellular Location:Nucleus
Calculated MW:52kDa
Observed MW:52kDa
Synonyms:GPKOW, GPATC5, GPATCH5, Spp2, T54
Background:This gene encodes a putative RNA-binding protein containing G-patch and KOW (Kyprides, Ouzounis, Woese) domains. The encoded protein interacts directly with protein kinase A and protein kinase X and is also found associated with the spliceosome.
UniProt Protein Function:GPKOW: a protein with G-patch and KOW domains which are involved in RNA binding. The encoded protein may be orthologous to the Arabidopsis protein, modifier of snc 1, 2, which contains the same domains and is required for innate resistance to some pathogens (PMID: 16271871). [provided by RefSeq, Aug 2011]
UniProt Protein Details:

Protein type:RNA-binding

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: nucleoplasm

Molecular Function:protein binding; nucleic acid binding

NCBI Summary:This gene encodes a putative RNA-binding protein containing G-patch and KOW (Kyprides, Ouzounis, Woese) domains. The encoded protein interacts directly with protein kinase A and protein kinase X and is also found associated with the spliceosome. [provided by RefSeq, Aug 2013]
UniProt Code:Q92917
NCBI GenInfo Identifier:134048659
NCBI Gene ID:27238
NCBI Accession:Q92917.2
UniProt Secondary Accession:Q92917,Q59EK5, Q9BQA8,
UniProt Related Accession:Q92917
Molecular Weight:476
NCBI Full Name:G patch domain and KOW motifs-containing protein
NCBI Synonym Full Names:G patch domain and KOW motifs
NCBI Official Symbol:GPKOW  
NCBI Official Synonym Symbols:T54; Spp2; GPATC5; GPATCH5  
NCBI Protein Information:G patch domain and KOW motifs-containing protein; protein MOS2 homolog
UniProt Protein Name:G patch domain and KOW motifs-containing protein
UniProt Synonym Protein Names:G patch domain-containing protein 5; Protein MOS2 homolog; Protein T54
Protein Family:G patch domain and KOW motifs-containing protein
UniProt Gene Name:GPKOW  
UniProt Entry Name:GPKOW_HUMAN
Anti-GPKOW Antibody (CAB13214)Western blot analysis of extracts of various cell lines, using GPKOW antibody (CAB13214) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s.
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