Metabolism Antibodies 1

Anti-GPI Antibody (CAB13308)

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SKU:
CAB13308
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-GPI Antibody
Antibody SKU:CAB13308
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human GPI (NP_000166.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, BT-474, MCF7, HeLa, Mouse kidney, Mouse heart, Mouse brain, Rat brain, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human GPI (NP_000166.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAL TRDP QFQK LQQW YREH RSEL NLRR LFDA NKDR FNHF SLTL NTNH GHIL VDYS KNLV TEDV MRML VDLA KSRG VEAA RERM FNGE KINY TEGR AVLH VALR NRSN TPIL VDGK DVMP EVNK VLDK MKSF CQRV RSGD WKGY TGKT ITDV INIG IGGS DLGP LMVT EALK PYSS GGPR VWYV SNID GTHI AKTL AQLN PESS LFII ASKT FTTQ ETIT NAET AKEW FLQA AKDP SAVA KHFV ALST NTTK VKEF GIDP QNMF EFWD WVGG RYSL WSAI
Gene ID:2821
Uniprot:P06744
Cellular Location:Cytoplasm, Secreted
Calculated MW:63kDa/64kDa
Observed MW:56kDa
Synonyms:GPI, AMF, GNPI, NLK, PGI, PHI, SA-36, SA36
Background:This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
UniProt Protein Details:

Protein type:Isomerase; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 5.3.1.9; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cytokine; Carbohydrate Metabolism - pentose phosphate pathway; Apoptosis

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: nucleoplasm; extracellular space; neuron projection; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function:monosaccharide binding; glucose-6-phosphate isomerase activity; growth factor activity; cytokine activity; intramolecular transferase activity

Biological Process: aldehyde catabolic process; methylglyoxal biosynthetic process; glycolysis; glucose 6-phosphate metabolic process; glucose metabolic process; pathogenesis; negative regulation of caspase activity; humoral immune response; gluconeogenesis; learning and/or memory; hemostasis; carbohydrate metabolic process; negative regulation of neuron apoptosis; angiogenesis

Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency

NCBI Summary:This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:P06744
NCBI GenInfo Identifier:17380385
NCBI Gene ID:2821
NCBI Accession:P06744.4
UniProt Secondary Accession:P06744,Q9BRD3, Q9BSK5, Q9UHE6, B4DG39,
UniProt Related Accession:P06744
Molecular Weight:558
NCBI Full Name:Glucose-6-phosphate isomerase
NCBI Synonym Full Names:glucose-6-phosphate isomerase
NCBI Official Symbol:GPI  
NCBI Official Synonym Symbols:AMF; NLK; PGI; PHI; GNPI; SA36; SA-36  
NCBI Protein Information:glucose-6-phosphate isomerase; neuroleukin; oxoisomerase; sperm antigen 36; sperm antigen-36; phosphohexomutase; phosphosaccharomutase; phosphohexose isomerase; phosphoglucose isomerase; autocrine motility factor; hexosephosphate isomerase; glucose phosph
UniProt Protein Name:Glucose-6-phosphate isomerase
UniProt Synonym Protein Names:Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI; Sperm antigen 36; SA-36
Protein Family:Glucose-6-phosphate isomerase
UniProt Gene Name:GPI  
UniProt Entry Name:G6PI_HUMAN
Anti-GPI Antibody (CAB13308)Western blot analysis of extracts of various cell lines, using GPI antibody (CAB13308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-GPI Antibody (CAB13308)Immunohistochemistry of paraffin-embedded rat brain using GPI antibody (CAB13308) at dilution of 1:200 (40x lens).
Anti-GPI Antibody (CAB13308)Immunohistochemistry of paraffin-embedded rat kidney using GPI antibody (CAB13308) at dilution of 1:200 (40x lens).
Anti-GPI Antibody (CAB13308)Immunofluorescence analysis of NIH-3T3 cells using GPI antibody (CAB13308) at dilution of 1:100. Blue: DAPI for nuclear staining.
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