Cell Biology Antibodies 10
Anti-GPD2 Antibody (CAB6611)
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- SKU:
- CAB6611
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GPD2 Antibody |
| Antibody SKU: | CAB6611 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-325 of human GPD2 (NP_000399.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, THP-1, SW480, Mouse skeletal muscle, Mouse liver, Mouse pancreas, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-325 of human GPD2 (NP_000399.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SQFA HYRR KQMN LAYV KAAD CISE PVNR EPPS REAQ LLTL QNTS EFDI LVIG GGAT GSGC ALDA VTRG LKTA LVER DDFS SGTS SRST KLIH GGVR YLQK AIMK LDIE QYRM VKEA LHER ANLL EIAP HLSA PLPI MLPV YKWW QLPY YWVG IKLY DLVA GSNC LKSS YVLS KSRA LEHF PMLQ KDKL VGAI VYYD GQHN DARM NLAI ALTA ARYG AATA NYME VVSL LKKT DPQT GKVR VSGA RCKD VLTG QEFD VRAK CVIN ATGP FTDS VRKM DDKD AAAI CQPS AGVH IVMP GYYS P |
| Gene ID: | 2820 |
| Uniprot: | P43304 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 67kDa/80kDa |
| Observed MW: | 81kDa |
| Synonyms: | GPD2, GDH2, GPDM, mGPDH |
| Background: | The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | GPD2: localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010] |
| UniProt Protein Details: | Protein type:Lipid Metabolism - glycerophospholipid; EC 1.1.5.3; Mitochondrial; Oxidoreductase; Calcium-binding Chromosomal Location of Human Ortholog: 2q24.1 Cellular Component: mitochondrial inner membrane Molecular Function:glycerol-3-phosphate dehydrogenase activity Biological Process: cellular lipid metabolic process Disease: Diabetes Mellitus, Noninsulin-dependent |
| NCBI Summary: | The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010] |
| UniProt Code: | P43304 |
| NCBI GenInfo Identifier: | 229462943 |
| NCBI Gene ID: | 2820 |
| NCBI Accession: | P43304.3 |
| UniProt Secondary Accession: | P43304,Q59FR1, Q9HAP9, A8K4V0, B3KSA9, |
| UniProt Related Accession: | P43304 |
| Molecular Weight: | 67,531 Da |
| NCBI Full Name: | Glycerol-3-phosphate dehydrogenase, mitochondrial |
| NCBI Synonym Full Names: | glycerol-3-phosphate dehydrogenase 2 |
| NCBI Official Symbol: | GPD2 |
| NCBI Official Synonym Symbols: | GDH2; GPDM; mGPDH |
| NCBI Protein Information: | glycerol-3-phosphate dehydrogenase, mitochondrial |
| UniProt Protein Name: | Glycerol-3-phosphate dehydrogenase, mitochondrial |
| UniProt Synonym Protein Names: | mtGPD |
| Protein Family: | Glycerol-3-phosphate dehydrogenase |
| UniProt Gene Name: | GPD2 |
| UniProt Entry Name: | GPDM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GPD2 antibody (CAB6611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human liver damage using GPD2 Antibody (CAB6611) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using GPD2 Antibody (CAB6611) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using GPD2 Antibody (CAB6611) at dilution of 1:100 (40x lens). |
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