Cell Biology Antibodies 1

Anti-GPC5 Antibody (CAB10411)

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SKU:
CAB10411
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GPC5 Antibody
Antibody SKU:CAB10411
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 320-550 of human GPC5 (NP_004457.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, BT-474, Mouse brain, Mouse lung, Rat brain, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 320-550 of human GPC5 (NP_004457.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HLLV NDAV LQAH LNGQ KLLE QVNR ICGR PVRT PTQS PRCS FDQS KEKH GMKT TTRN SEET LANR RKEF INSL RLYR SFYG GLAD QLCA NELA AADG LPCW NGED IVKS YTQR VVGN GIKA QSGN PEVK VKGI DPVI NQII DKLK HVVQ LLQG RSPK PDKW ELLQ LGSG GGMV EQVS GDCD DEDG CGGS GSGE VKRT LKIT DWMP DDMN FSDV KQIH QTDT GSTL DTTG AGC
Gene ID:2262
Uniprot:P78333
Cellular Location:Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, Secreted, extracellular space
Calculated MW:63kDa
Observed MW:72kDa
Synonyms:GPC5
Background:Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
UniProt Protein Function:GPC5: Cell surface proteoglycan that bears heparan sulfate. Belongs to the glypican family.
UniProt Protein Details:

Protein type:Cell surface; Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q31.3

Cellular Component: extracellular region; Golgi lumen; integral to plasma membrane; lysosomal lumen; plasma membrane

Biological Process: glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; retinoid metabolic process

NCBI Summary:Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]
UniProt Code:P78333
NCBI GenInfo Identifier:2829667
NCBI Gene ID:2262
NCBI Accession:P78333.1
UniProt Secondary Accession:P78333,O60436, Q9BX27, B2R726,
UniProt Related Accession:P78333
Molecular Weight:64kDa
NCBI Full Name:Glypican-5
NCBI Synonym Full Names:glypican 5
NCBI Official Symbol:GPC5  
NCBI Protein Information:glypican-5
UniProt Protein Name:Glypican-5
Protein Family:Glypican
UniProt Gene Name:GPC5  
Anti-GPC5 Antibody (CAB10411)Western blot analysis of extracts of various cell lines, using GPC5 antibody (CAB10411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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