Neuroscience
Anti-GPC4 Antibody (CAB12805)
- SKU:
- CAB12805
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Neuroscience
Description
| Antibody Name: | Anti-GPC4 Antibody |
| Antibody SKU: | CAB12805 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 357-556 of human GPC4 (NP_001439.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, A-431, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 357-556 of human GPC4 (NP_001439.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SESA FSAR FRPH HPEE RPTT AAGT SLDR LVTD VKEK LKQA KKFW SSLP SNVC NDER MAAG NGNE DDCW NGKG KSRY LFAV TGNG LANQ GNNP EVQV DTSK PDIL ILRQ IMAL RVMT SKMK NAYN GNDV DFFD ISDE SSGE GSGS GCEY QQCP SEFD YNAT DHAG KSAN EKAD SAGV RPGA QAYL LTVF CILF LVMQ REWR |
| Gene ID: | 2239 |
| Uniprot: | O75487 |
| Cellular Location: | Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, Secreted, extracellular space |
| Calculated MW: | 54kDa/62kDa |
| Observed MW: | 70kDa |
| Synonyms: | GPC4, K-glypican |
| Background: | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. |
| UniProt Protein Function: | GPC4: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. Belongs to the glypican family. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: Xq26.2 Cellular Component: external side of plasma membrane; Golgi lumen; integral to plasma membrane; lysosomal lumen; nucleus; plasma membrane Biological Process: anatomical structure morphogenesis; cell proliferation; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; retinoid metabolic process Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1 |
| NCBI Summary: | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75487 |
| NCBI GenInfo Identifier: | 30923161 |
| NCBI Gene ID: | 2239 |
| NCBI Accession: | O75487.4 |
| UniProt Secondary Accession: | O75487,Q6ZMA6, Q96L43, Q9NU08, Q9UJN1, Q9UPD9, B2R6J7 B4E2C0, |
| UniProt Related Accession: | O75487 |
| Molecular Weight: | 62kDa |
| NCBI Full Name: | Glypican-4 |
| NCBI Synonym Full Names: | glypican 4 |
| NCBI Official Symbol: | GPC4 |
| NCBI Official Synonym Symbols: | K-glypican |
| NCBI Protein Information: | glypican-4 |
| UniProt Protein Name: | Glypican-4 |
| UniProt Synonym Protein Names: | K-glypican |
| Protein Family: | Glypican |
| UniProt Gene Name: | GPC4 |
 | Western blot analysis of extracts of various cell lines, using GPC4 antibody (CAB12805) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human liver using GPC4 antibody (CAB12805) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using GPC4 antibody (CAB12805) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using GPC4 antibody (CAB12805) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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