Cell Biology Antibodies 9
Anti-GOSR1 Antibody (CAB4316)
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- SKU:
- CAB4316
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GOSR1 Antibody |
| Antibody SKU: | CAB4316 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human GOSR1 (NP_004862.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human GOSR1 (NP_004862.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAG TSSY WEDL RKQA RQLE NELD LKLV SFSK LCTS YSHS STRD GRRD RYSS DTTP LLNG SSQD RMFE TMAI EIEQ LLAR LTGV NDKM AEYT NSAG VPSL NAAL MHTL QRHR DIL |
| Gene ID: | 9527 |
| Uniprot: | O95249 |
| Cellular Location: | Golgi apparatus membrane, Single-pass type IV membrane protein |
| Calculated MW: | 21kDa/28kDa |
| Observed MW: | 29kDa |
| Synonyms: | GOSR1, GOLIM2, GOS-28, GOS28, GOS28/P28, GS28, P28 |
| Background: | This gene encodes a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments. This protein is considered an essential component of the Golgi SNAP receptor (SNARE) complex. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | GOSR1: a single-pass type IV Golgi membrane protein. Enriched on vesicular components at the terminal rims of the Golgi. Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. Identified in a unique SNARE complex composed of the Golgi SNAREs GOSR2, STX5, GABARAPL2 and YKT6. |
| UniProt Protein Details: | Protein type:Vesicle; Membrane protein, integral; Autophagy Chromosomal Location of Human Ortholog: 17q11 Cellular Component: Golgi membrane; SNARE complex; Golgi apparatus; cis-Golgi network; membrane; integral to membrane Molecular Function:SNAP receptor activity Biological Process: intra-Golgi vesicle-mediated transport; protein transport; ER to Golgi vesicle-mediated transport; retrograde transport, endosome to Golgi |
| NCBI Summary: | This gene encodes a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments. This protein is considered an essential component of the Golgi SNAP receptor (SNARE) complex. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95249 |
| NCBI GenInfo Identifier: | 12643659 |
| NCBI Gene ID: | 9527 |
| NCBI Accession: | O95249.1 |
| UniProt Secondary Accession: | O95249,O75392, J3KST5, |
| UniProt Related Accession: | O95249 |
| Molecular Weight: | 250 |
| NCBI Full Name: | Golgi SNAP receptor complex member 1 |
| NCBI Synonym Full Names: | golgi SNAP receptor complex member 1 |
| NCBI Official Symbol: | GOSR1 |
| NCBI Official Synonym Symbols: | P28; GS28; GOS28; GOLIM2; GOS-28; GOS28/P28 |
| NCBI Protein Information: | Golgi SNAP receptor complex member 1; cis-golgi SNARE; Golgi SNARE 28 kDa; 28 kDa Golgi SNARE protein; 28 kDa cis-Golgi SNARE p28; golgi integral membrane protein 2 |
| UniProt Protein Name: | Golgi SNAP receptor complex member 1 |
| UniProt Synonym Protein Names: | 28 kDa Golgi SNARE protein; 28 kDa cis-Golgi SNARE p28; GOS-28 |
| UniProt Gene Name: | GOSR1 |
| UniProt Entry Name: | GOSR1_HUMAN |
 | Western blot analysis of extracts of mouse testis, using GOSR1 antibody (CAB4316) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 15s. |
 | Immunofluorescence analysis of U2OS cells using GOSR1 antibody (CAB4316) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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