Name: Anti-GORASP2 Antibody (CAB10383)
Brand: Developmental Biology
SKU: CAB10383
Availability: OutOfStock

Antibody Name:	Anti-GORASP2 Antibody
Antibody SKU:	CAB10383
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 353-452 of human GORASP2 (NP_056345.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, HeLa, Jurkat, MCF7, K-562, SW480, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 353-452 of human GORASP2 (NP_056345.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PGIA PLPL PSEF LPSF PLVP ESSS AASS GELL SSLP PTSN APSD PATT TAKA DAAS SLTV DVTP PTAK APTT VEDR VGDS TPVS EKPV SAAV DANA SESP
Gene ID:	26003
Uniprot:	Q9H8Y8
Cellular Location:	Golgi apparatus membrane, Lipid-anchor, Membrane, Peripheral membrane protein
Calculated MW:	39kDa/47kDa/48kDa
Observed MW:	55kDa

Synonyms:

GORASP2, GOLPH6, GRASP55, GRS2, p59

Background:

This gene encodes a member of the Golgi reassembly stacking protein family. These proteins may play a role in the stacking of Golgi cisternae and Golgi ribbon formation, as well as Golgi fragmentation during apoptosis or mitosis. The encoded protein also plays a role in the intracellular transport of transforming growth factor alpha and may function as a molecular chaperone. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	GRASP55: a golgi reassembly-stacking protein (GRASP) protein involved in the stacking of Golgi cisternae. May be involved in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as the TGF-alpha receptor. Oligomerization of its N-terminal GRASP domain regulates the formation of Golgi stacks, a process that is regulated by phosphorylation within the C-terminal Ser/Pro-rich domain. Forms a RAB2 effector complex with BLZF1 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of the TGF-alpha receptor, prior its transit in the trans-Golgi. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Vesicle; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2q31.1 Cellular Component: Golgi membrane; Golgi apparatus; membrane Molecular Function:protein binding Biological Process: organelle organization and biogenesis; mitotic cell cycle
NCBI Summary:	This gene encodes a member of the Golgi reassembly stacking protein family. These proteins may play a role in the stacking of Golgi cisternae and Golgi ribbon formation, as well as Golgi fragmentation during apoptosis or mitosis. The encoded protein also plays a role in the intracellular transport of transforming growth factor alpha and may function as a molecular chaperone. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:	Q9H8Y8
NCBI GenInfo Identifier:	51316097
NCBI Gene ID:	26003
NCBI Accession:	Q9H8Y8.3
UniProt Secondary Accession:	Q9H8Y8,Q53TE3, Q96I74, Q96K84, Q9H946, Q9UFW4, B4DKT0
UniProt Related Accession:	Q9H8Y8
Molecular Weight:	48,674 Da
NCBI Full Name:	Golgi reassembly-stacking protein 2
NCBI Synonym Full Names:	golgi reassembly stacking protein 2, 55kDa
NCBI Official Symbol:	GORASP2
NCBI Official Synonym Symbols:	p59; GRS2; GOLPH6; GRASP55
NCBI Protein Information:	Golgi reassembly-stacking protein 2; golgi phosphoprotein 6
UniProt Protein Name:	Golgi reassembly-stacking protein 2
UniProt Synonym Protein Names:	Golgi phosphoprotein 6; GOLPH6; Golgi reassembly-stacking protein of 55 kDa; GRASP55; p59
Protein Family:	Golgi reassembly-stacking protein
UniProt Gene Name:	GORASP2
UniProt Entry Name:	GORS2_HUMAN

Western blot analysis of extracts of various cell lines, using GORASP2 antibody (CAB10383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

GORASP2 Antibody (PACO00883)

GORASP2 Antibody (PACO09537)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)