Cell Biology Antibodies 11
Anti-GOLM1 Antibody (CAB7283)
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- SKU:
- CAB7283
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GOLM1 Antibody |
| Antibody SKU: | CAB7283 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 112-401 of human GOLM1 (NP_057632.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 112-401 of human GOLM1 (NP_057632.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TGER LIRV LQDQ LKTL QRNY GRLQ QDVL QFQK NQTN LERK FSYD LSQC INQM KEVK EQCE ERIE EVTK KGNE AVAS RDLS ENND QRQQ LQAL SEPQ PRLQ AAGL PHTE VPQG KGNV LGNS KSQT PAPS SEVV LDSK RQVE KEET NEIQ VVNE EPQR DRLP QEPG REQV VEDR PVGG RGFG GAGE LGQT PQVQ AALS VSQE NPEM EGPE RDQL VIPD GQEE EQEA AGEG RNQQ KLRG EDDY NMDE NEAE SETD KQAA LAGN DRNI DVFN VEDQ KRDT INLL DQRE KRNH TL |
| Gene ID: | 51280 |
| Uniprot: | Q8NBJ4 |
| Cellular Location: | Golgi apparatus, Single-pass type II membrane protein, cis-Golgi network membrane |
| Calculated MW: | 44kDa/45kDa |
| Observed MW: | 75KDa |
| Synonyms: | GOLM1, C9orf155, GOLPH2, GP73, HEL46, PSEC0257, bA379P1.3 |
| Background: | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. |
| UniProt Protein Function: | GOLM1: Unknown. Cellular response protein to viral infection. Belongs to the GOLM1/CASC4 family. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 9q21.33 Cellular Component: Golgi apparatus; extracellular space; integral to plasma membrane Molecular Function:protein binding Biological Process: regulation of lipid metabolic process; nuclear organization and biogenesis |
| UniProt Code: | Q8NBJ4 |
| NCBI GenInfo Identifier: | 51316027 |
| NCBI Gene ID: | |
| NCBI Accession: | Q8NBJ4.1 |
| Molecular Weight: | 45kDa |
| NCBI Full Name: | Golgi membrane protein 1 |
| UniProt Protein Name: | Golgi membrane protein 1 |
| UniProt Synonym Protein Names: | Golgi membrane protein GP73; Golgi phosphoprotein 2 |
| Protein Family: | Golgi membrane protein |
| UniProt Gene Name: | GOLM1 |
| UniProt Entry Name: | GOLM1_HUMAN |
 | Western blot analysis of extracts of Rat lung, using GOLM1 antibody (CAB7283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat brain using GOLM1 Rabbit pAb (CAB7283) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using GOLM1 Rabbit pAb (CAB7283) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using GOLM1 antibody (CAB7283) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining. |
 | Confocal immunofluorescence analysis of Hela cells using GOLM1 antibody (CAB7283) at dilution of 1:200. Blue: DAPI for nuclear staining. |
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