Antibody Name:	Anti-GOLGA5 Antibody
Antibody SKU:	CAB15768
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA5 (NP_005104.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, Jurkat, HeLa, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA5 (NP_005104.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSWF VDLA GKAE DLLN RVDQ GAAT ALSR KDNA SNIY SKNT DYTE LHQQ NTDL IYQT GPKS TYIS SAAD NIRN QKAT ILAG TANV KVGS RTPV EASH PVEN ASVP RPSS HFVR RKKS EPDD ELLF DFLN SSQK EPTG RVEI RKEK GKTP VFQS SQTS SVSS VNPS VTTI KTIE ENSF GSQT HEAA SNSD SSHE GQEE SSKE NVSS NAAC PDHT PTPN DDGK SHEL SNLR LENQ LLRN EVQS LNQE MASL LQRS KETQ EELN KARA RVEK WNAD HSKS DRMT RGLR AQVD DLTE AVAA KDSQ
Gene ID:	9950
Uniprot:	Q8TBA6
Cellular Location:	Golgi apparatus membrane, Single-pass type IV membrane protein
Calculated MW:	78kDa/83kDa
Observed MW:	100kDa, 115kDa

Synonyms:

GOLGA5, GOLIM5, RFG5, ret-II, golgin A5

Background:

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5.

UniProt Protein Function:	GOLGA5: a member of the golgin family of proteins, whose members localize to the Golgi. Involved in regulating the architecture of the Golgi apparatus. A coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues. Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase. Two splice variant isoforms have been described.
UniProt Protein Details:	Protein type:Cytoskeletal; Membrane protein, integral Chromosomal Location of Human Ortholog: 14q32.12 Cellular Component: cis-Golgi network; Golgi apparatus; Golgi cisterna; Golgi membrane; integral to membrane; membrane; transport vesicle Molecular Function:protein homodimerization activity; Rab GTPase binding Biological Process: Golgi organization and biogenesis; Golgi vesicle transport; retrograde transport, vesicle recycling within Golgi Disease: Thyroid Carcinoma, Papillary
NCBI Summary:	The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. [provided by RefSeq, Jul 2013]
UniProt Code:	Q8TBA6
NCBI GenInfo Identifier:	296439337
NCBI Gene ID:	9950
NCBI Accession:	Q8TBA6.3
UniProt Secondary Accession:	Q8TBA6,O95287, Q03962, Q2TS49, Q9UQQ7, C9JRU1,
UniProt Related Accession:	Q8TBA6
Molecular Weight:	78,108 Da
NCBI Full Name:	Golgin subfamily A member 5
NCBI Synonym Full Names:	golgin A5
NCBI Official Symbol:	GOLGA5
NCBI Official Synonym Symbols:	RFG5; GOLIM5; ret-II
NCBI Protein Information:	golgin subfamily A member 5
UniProt Protein Name:	Golgin subfamily A member 5
UniProt Synonym Protein Names:	Cell proliferation-inducing gene 31 protein; Golgin-84; Protein Ret-II; RET-fused gene 5 protein
Protein Family:	Golgin subfamily
UniProt Gene Name:	GOLGA5
UniProt Entry Name:	GOGA5_HUMAN

	Western blot analysis of extracts of various cell lines, using GOLGA5 antibody (CAB15768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of C6 cells using GOLGA5 antibody (CAB15768) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using GOLGA5 antibody (CAB15768) at dilution of 1:100. Blue: DAPI for nuclear staining.