Cell Biology Antibodies 1

Anti-GOLGA4 Antibody (CAB10216)

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SKU:
CAB10216
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GOLGA4 Antibody
Antibody SKU:CAB10216
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GOLGA4 (NP_002069.2).
Application:WB IHC IF
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HeLa, SW480, A-549, MCF7, Mouse heart, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GOLGA4 (NP_002069.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MFKK LKQK ISEE QQQL QQAL APAQ ASSN SSTP TRMR SRTS SFTE QLDE GTPN RESG DTQS FAQK LQLR VPSV ESLF RSPI KESL FRSS SKES LVRT SSRE SLNR LDLD SSTA SFDP PSDM DSEA EDLV GNSD SLNK EQLI QRLR RMER SL
Gene ID:2803
Uniprot:Q13439
Cellular Location:Cytoplasm, Golgi apparatus, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:260kDa/261kDa/262kDa
Observed MW:280kDa
Synonyms:GOLGA4, CRPF46, GCP2, GOLG, MU-RMS-40.18, p230, golgin A4
Background:The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein has been postulated to play a role in Rab6-regulated membrane-tethering events in the Golgi apparatus. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
UniProt Protein Function:GOLGA4: May play a role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with MACF1. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle

Chromosomal Location of Human Ortholog: 3p22.2

Cellular Component: cytoplasm; cytosol; Golgi apparatus; intracellular membrane-bound organelle; trans-Golgi network

Molecular Function:GTPase binding; protein binding

Biological Process: Golgi to plasma membrane protein transport; positive regulation of axon extension; vesicle-mediated transport

NCBI Summary:The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein has been postulated to play a role in Rab6-regulated membrane-tethering events in the Golgi apparatus. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010]
UniProt Code:Q13439
NCBI GenInfo Identifier:12643718
NCBI Gene ID:2803
NCBI Accession:Q13439.1
UniProt Secondary Accession:Q13439,Q13270, Q13654, Q14436, Q59EW8, F8W8Q7,
UniProt Related Accession:Q13439
Molecular Weight:261kDa
NCBI Full Name:Golgin subfamily A member 4
NCBI Synonym Full Names:golgin A4
NCBI Official Symbol:GOLGA4  
NCBI Official Synonym Symbols:GCP2; GOLG; p230; CRPF46; MU-RMS-40.18  
NCBI Protein Information:golgin subfamily A member 4
UniProt Protein Name:Golgin subfamily A member 4
UniProt Synonym Protein Names:256 kDa golgin; Golgin-245; Protein 72.1; Trans-Golgi p230
Protein Family:Golgin subfamily
UniProt Gene Name:GOLGA4  
Anti-GOLGA4 Antibody (CAB10216)Western blot analysis of extracts of various cell lines, using GOLGA4 antibody (CAB10216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-GOLGA4 Antibody (CAB10216)Immunofluorescence analysis of C6 cells using GOLGA4 Rabbit pAb (CAB10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-GOLGA4 Antibody (CAB10216)Immunofluorescence analysis of HeLa cells using GOLGA4 Rabbit pAb (CAB10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-GOLGA4 Antibody (CAB10216)Immunofluorescence analysis of L929 cells using GOLGA4 Rabbit pAb (CAB10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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