Name: Anti-GNPAT Antibody (CAB4220)
Brand: Cell Biology Antibodies 9
SKU: CAB4220
Availability: OutOfStock

Antibody Name:	Anti-GNPAT Antibody
Antibody SKU:	CAB4220
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 481-680 of human GNPAT (NP_055051.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human
Positive Samples:	U-87MG, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 481-680 of human GNPAT (NP_055051.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YRNQ LLNI FVRP SLVA VALQ MTPG FRKE DVYS CFRF LRDV FADE FIFL PGNT LKDF EEGC YLLC KSEA IQVT TKDI LVTE KGNT VLEF LVGL FKPF VESY QIIC KYLL SEEE DHFS EEQY LAAV RKFT SQLL DQGT SQCY DVLS SDVQ KNAL AACV RLGV VEKK KINN NCIF NVNE PATT KLEE MLGC KTPI GKPA TAKL
Gene ID:	8443
Uniprot:	O15228
Cellular Location:	Matrix side, Peripheral membrane protein, Peroxisome membrane
Calculated MW:	69kDa/77kDa
Observed MW:	70kDa

Synonyms:	GNPAT, DAP-AT, DAPAT, DHAPAT, RCDP2
Background:	This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GNPAT: Defects in GNPAT are the cause of rhizomelic chondrodysplasia punctata type 2 (RCDP2). RDCP2 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation. Belongs to the GPAT/DAPAT family.
UniProt Protein Details:	Protein type:EC 2.3.1.42; Lipid Metabolism - glycerophospholipid; Transferase Chromosomal Location of Human Ortholog: 1q42 Cellular Component: peroxisomal membrane; peroxisomal matrix; mitochondrion; membrane; peroxisome Molecular Function:glycerone-phosphate O-acyltransferase activity; palmitoyl-CoA hydrolase activity; receptor binding Biological Process: response to drug; response to starvation; synaptogenesis; phospholipid metabolic process; glycerophospholipid biosynthetic process; cerebellum morphogenesis; phosphatidic acid biosynthetic process; cellular lipid metabolic process; response to nutrient; ether lipid biosynthetic process; paranodal junction assembly Disease: Rhizomelic Chondrodysplasia Punctata, Type 2
NCBI Summary:	This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. [provided by RefSeq, Jul 2008]
UniProt Code:	O15228
NCBI GenInfo Identifier:	3913409
NCBI Gene ID:	8443
NCBI Accession:	O15228.1
UniProt Secondary Accession:	O15228,Q5TBH7, Q9BWC2, B4DNM9,
UniProt Related Accession:	O15228
Molecular Weight:	680
NCBI Full Name:	Dihydroxyacetone phosphate acyltransferase
NCBI Synonym Full Names:	glyceronephosphate O-acyltransferase
NCBI Official Symbol:	GNPAT
NCBI Official Synonym Symbols:	DAPAT; DAP-AT; DHAPAT
NCBI Protein Information:	dihydroxyacetone phosphate acyltransferase; DHAP-AT; glycerone-phosphate O-acyltransferase; acyl-CoA:dihydroxyacetonephosphateacyltransferase
UniProt Protein Name:	Dihydroxyacetone phosphate acyltransferase
UniProt Synonym Protein Names:	Acyl-CoA:dihydroxyacetonephosphateacyltransferase; Glycerone-phosphate O-acyltransferase
Protein Family:	Dihydroxyacetone phosphate acyltransferase
UniProt Gene Name:	GNPAT
UniProt Entry Name:	GNPAT_HUMAN

Western blot analysis of extracts of various cell lines, using GNPAT antibody (CAB4220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

GNPAT Antibody (PACO05520)

GNPAT Antibody (PACO09526)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)