Cell Biology Antibodies 10

Anti-GNMT Antibody (CAB6608)

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SKU:
CAB6608
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GNMT Antibody
Antibody SKU:CAB6608
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human GNMT (NP_061833.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse liver, Mouse kidney, Mouse pancreas, Rat liver, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human GNMT (NP_061833.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVDS VYRT RSLG VAAE GLPD QYAD GEAA RVWQ LYIG DTRS RTAE YKAW LLGL LRQH GCQR VLDV ACGT GVDS IMLV EEGF SVTS VDAS DKML KYAL KERW NRRH EPAF DKWV IEEA NWMT LDKD VPQS AEGG FDAV ICLG NSFA HLPD CKGD QSEH RLAL KNIA SMVR AGGL LVID HRNY DHIL STGC APPG KNIY YKSD LTKD VTTS VLIV NNKA HMVT LDYT VQVP GAGQ DGSP GLSK FRLS YYPH CLAS FTEL LQAA FGGK CQHS VLGD FKPY KPGQ TYIP CYFI HVLK RTD
Gene ID:27232
Uniprot:Q14749
Cellular Location:Cytoplasm
Calculated MW:32kDa
Observed MW:33kDa
Synonyms:GNMT, HEL-S-182mP
Background:The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. This protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). Alternative splicing results in multiple transcript variants. Naturally occurring readthrough transcription occurs between the upstream CNPY3 (canopy FGF signaling regulator 3) gene and this gene and is represented with GeneID:107080644.
UniProt Protein Function:GNMT: Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Defects in GNMT are the cause of glycine N- methyltransferase deficiency (GNMT deficiency); also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases. Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; EC 2.1.1.20

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: cytoplasm

Molecular Function:protein binding; glycine N-methyltransferase activity; glycine binding; folic acid binding

Biological Process: methylation; glycogen metabolic process; S-adenosylmethionine metabolic process; protein modification process; regulation of gluconeogenesis; methionine metabolic process; one-carbon compound metabolic process; protein homotetramerization

Disease: Glycine N-methyltransferase Deficiency

NCBI Summary:The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). [provided by RefSeq, Oct 2008]
UniProt Code:Q14749
NCBI GenInfo Identifier:12644416
NCBI Gene ID:27232
NCBI Accession:Q14749.3
UniProt Secondary Accession:Q14749,Q5T8W2, Q9NNZ1, Q9NS24,
UniProt Related Accession:Q14749
Molecular Weight:295
NCBI Full Name:Glycine N-methyltransferase
NCBI Synonym Full Names:glycine N-methyltransferase
NCBI Official Symbol:GNMT  
NCBI Protein Information:glycine N-methyltransferase; HEL-S-182mP; epididymis secretory sperm binding protein Li 182mP
UniProt Protein Name:Glycine N-methyltransferase
Protein Family:Glycine N-methyltransferase
UniProt Gene Name:GNMT  
UniProt Entry Name:GNMT_HUMAN
Anti-GNMT Antibody (CAB6608)Western blot analysis of extracts of various cell lines, using GNMT antibody (CAB6608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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