Cell Biology Antibodies 4
Anti-GNB3 Antibody (CAB1387)
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- SKU:
- CAB1387
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GNB3 Antibody |
| Antibody SKU: | CAB1387 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human GNB3 (NP_002066.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse eye, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human GNB3 (NP_002066.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGEM EQLR QEAE QLKK QIAD ARKA CADV TLAE LVSG LEVV GRVQ MRTR RTLR GHLA KIYA MHWA TDSK LLVS ASQD GKLI VWDS YTTN KVHA IPLR SSWV MTCA YAPS GNFV ACGG LDNM CSIY NLKS REGN VKVS RELS AHTG YLSC CRFL DDNN IVTS SGDT TCAL WDIE TGQQ KTVF VGHT GDCM SLAV SPDF NLFI SGAC DASA KLWD VREG TCRQ TFTG HESD IN |
| Gene ID: | 2784 |
| Uniprot: | P16520 |
| Cellular Location: | |
| Calculated MW: | 32kDa/37kDa |
| Observed MW: | 37kDa |
| Synonyms: | GNB3, CSNB1H |
| Background: | Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. |
| UniProt Protein Function: | G-beta 3: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. G proteins are composed of 3 units, alpha, beta and gamma. Interacts with RASD2. Belongs to the WD repeat G protein beta family. |
| UniProt Protein Details: | Protein type:G protein; G protein, heterotrimeric beta; G protein, heterotrimeric Chromosomal Location of Human Ortholog: 12p13 Cellular Component: neuron projection; plasma membrane Molecular Function:GTPase activity; protein binding; GTPase binding; signal transducer activity; spectrin binding Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; regulation of blood pressure; energy reserve metabolic process Disease: Hypertension, Essential |
| NCBI Summary: | Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014] |
| UniProt Code: | P16520 |
| NCBI GenInfo Identifier: | 121011 |
| NCBI Gene ID: | 2784 |
| NCBI Accession: | P16520.1 |
| UniProt Related Accession: | P16520 |
| Molecular Weight: | 37kDa |
| NCBI Full Name: | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 |
| NCBI Synonym Full Names: | G protein subunit beta 3 |
| NCBI Official Symbol: | GNB3 |
| NCBI Official Synonym Symbols: | CSNB1H |
| NCBI Protein Information: | guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 |
| UniProt Protein Name: | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 |
| UniProt Synonym Protein Names: | Transducin beta chain 3 |
| UniProt Gene Name: | GNB3 |
| UniProt Entry Name: | GBB3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GNB3 antibody (CAB1387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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