Name: Anti-GNAI2 Antibody (CAB20930)
SKU: CAB20930
Availability: OutOfStock

Antibody Name:	Anti-GNAI2 Antibody (CAB20930)
Antibody SKU:	CAB20930
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunohistochemistry
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of Human GNAI2.

Application:	Western blotting, Immunohistochemistry
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-937, Mouse spleen, Mouse thymus, Rat lung, Rat thymus

Immunogen:	Recombinant protein of Human GNAI2.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Cellular Location:	Cell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	34kDa/36kDa/38kDa/40kDa/41kDa
Observed MW:	40KDa

Synonyms:	GNAI2, GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
Background:	The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene.

	Immunohistochemistry of paraffin-embedded human liver using GNAI2 Rabbit mAb at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human lung cancer using GNAI2 Rabbit mAb at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Western blot analysis of extracts of various cell lines, using GNAI2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.