Name: Anti-GNA13 Antibody (CAB5746)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB5746
Availability: OutOfStock

Antibody Name:	Anti-GNA13 Antibody [KO Validated]
Antibody SKU:	CAB5746
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human GNA13

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, BT-474, Jurkat, Mouse kidney, Mouse lung, Mouse spleen, Rat kidney

Immunogen:	Recombinant protein of human GNA13
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	10672
Uniprot:	Q14344
Cellular Location:	Cytoplasm, Lipid-anchor, Melanosome, Membrane, Nucleus
Calculated MW:	33kDa/44kDa
Observed MW:	44kDa

Synonyms:	GNA13, G13
Background:

UniProt Protein Function:	G-alpha 13: a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Activates Rho. Protein kinase A blocks Rho activation by phosphorylation of G-alpha(13). Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Regulates cytoplasmic as well as nuclear signaling events such as activation of the Jun N-terminal kinase signaling module, Na+/H+ exchangers, focal adhesion assemblies, and transcriptional activation of specific primary response genes.
UniProt Protein Details:	Protein type:G protein; G protein, heterotrimeric; G protein, heterotrimeric alpha G(12) Chromosomal Location of Human Ortholog: 17q24.3 Cellular Component: brush border membrane; cytoplasm; focal adhesion; heterotrimeric G-protein complex; membrane; plasma membrane Molecular Function:D5 dopamine receptor binding; G-protein beta/gamma-subunit binding; GTPase activity; protein binding; signal transducer activity; type 1 angiotensin receptor binding Biological Process: cell motility; G-protein signaling, adenylate cyclase activating pathway; phospholipase D activation; platelet activation; Rho protein signal transduction; signal transduction
UniProt Code:	Q14344
NCBI GenInfo Identifier:	38258936
NCBI Gene ID:	10672
NCBI Accession:	Q14344.2
UniProt Secondary Accession:	Q14344,Q8TD70, B2R977, B7Z7R0, F5H1G8,
UniProt Related Accession:	Q14344
Molecular Weight:	33,287 Da
NCBI Full Name:	Guanine nucleotide-binding protein subunit alpha-13
NCBI Synonym Full Names:	G protein subunit alpha 13
NCBI Official Symbol:	GNA13
NCBI Official Synonym Symbols:	G13
NCBI Protein Information:	guanine nucleotide-binding protein subunit alpha-13
UniProt Protein Name:	Guanine nucleotide-binding protein subunit alpha-13
Protein Family:	Guanine nucleotide-binding protein
UniProt Gene Name:	GNA13
UniProt Entry Name:	GNA13_HUMAN

	Western blot analysis of extracts of various cell lines, using GNA13 antibody (CAB5746) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and GNA13 knockout (KO) HeLa cells, using GNA13 antibody (CAB5746) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human stomach using GNA13 antibody (CAB5746) (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using GNA13 antibody (CAB5746) (40x lens).
	Immunofluorescence analysis of C6 cells using GNA13 Polyclonal Antibody (CAB5746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.