Description
Antibody Name: | Anti-Gn1a Antibody (CAB20679) |
Antibody SKU: | CAB20679 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Oryza sativa |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Oryza sativa Gn1a. |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Oryza sativa |
Positive Samples: | stems, spikes |
Immunogen: | Recombinant protein of Oryza sativa Gn1a. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | |
Calculated MW: | 60kDa |
Observed MW: | 70kDa |
Synonyms: | Gn1a, OsCKX2 |
Background: | is a gene for cytokinin oxidase/dehydrogenase (OsCKX2), an enzyme that degrades the phytohormone cytokinin.Reduced expression of OsCKX2 causes cytokinin accumulation in inflorescence meristems and increases the number of reproductive organs, resulting in enhanced grain yield. |
Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using Gn1a antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |