Cell Biology Antibodies 11
Anti-Gm13125 Antibody (CAB8095)
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- SKU:
- CAB8095
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Gm13125 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8095 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 67-274 of mouse Gm13125 (NP_001108549.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse ovary, Mouse heart, Mouse skeletal muscle, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 67-274 of mouse Gm13125 (NP_001108549.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GALK KRTP YLET QLQV VLEE VDKL LIQE VHPR EYKL EVLD LRSV GQNY LNVW PGAM DDWL PKTT QTDP CCSK TVMT QPLK VLVD LQLN NRGQ SRLF SYLV QWSD RRKG LLQL YCNK LQIW SPSQ QNYR KLSQ KVNL DYVE TLGL HSSC SPSF LLNF APYL RQMR NLRK LALS NIRE EHII SPEK RRCI ITRF TLQI LKLE SLQK LHLD |
| Gene ID: | 627009 |
| Uniprot: | Q5VT98 |
| Cellular Location: | |
| Calculated MW: | 55kDa |
| Observed MW: | 55kDa |
| Synonyms: | PRAMEF20, PRAMEF21, Gm13125 |
| Background: |
| UniProt Protein Function: | PRAMEF20: Belongs to the PRAME family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 1p36.21 Molecular Function:retinoic acid receptor binding Biological Process: negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell differentiation; positive regulation of cell proliferation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis |
| UniProt Code: | Q5VT98 |
| NCBI GenInfo Identifier: | 169234924 |
| NCBI Gene ID: | 627009 |
| NCBI Accession: | NP_001108549.1 |
| Molecular Weight: | Calculated: 55kDaObserved: 55kDa |
| NCBI Full Name: | novel preferentially expressed antigen in melanoma (Prame) protein |
| NCBI Synonym Full Names: | PRAME family member 20 |
| NCBI Official Symbol: | Pramef20 |
| NCBI Official Synonym Symbols: | Gm13125; EG627009 |
| NCBI Protein Information: | novel preferentially expressed antigen in melanoma (Prame) protein |
| UniProt Protein Name: | PRAME family member 20/21 |
| UniProt Gene Name: | PRAMEF20 |
| UniProt Entry Name: | PRA20_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Gm13125 antibody (CAB8095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse ovary using Gm13125 Rabbit pAb (CAB8095) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat ovary using Gm13125 Rabbit pAb (CAB8095) at dilution of 1:100 (40x lens). |
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