Cell Biology Antibodies 3
Anti-Gm13125 Antibody (CAB12619)
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- SKU:
- CAB12619
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Gm13125 Antibody |
| Antibody SKU: | CAB12619 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-477 of mouse Gm13125 (NP_001108549.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-477 of mouse Gm13125 (NP_001108549.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSSK PLLT LQEL AAQN ILRN EALA ISAL QYLP ELFL PQLL KQAY DGNH LKVL RAIV SSWP FPRL PLGA LKKR TPYL ETQL QVVL EEVD KLLI QEVH PREY KLEV LDLR SVGQ NYLN VWPG AMDD WLPK TTQT DPCC SKTV MTQP LKVL VDLQ LNNR GQSR LFSY LVQW SDRR KGLL QLYC NKLQ IWSP SQQN YRKL SQKV NLDY VETL GLHS SCSP SFLL NFAP YLRQ MRNL RKLA LSNI REEH IISP EKRR CIIT RFTL QILK LESL QKLH LDAV CFLE GHLD QLLG SVKT SLDE LAVT HCNL SVSE WSHF SEFP CVSQ LQQL NLEN VRLT GLSP EPLQ VLLV KAGP TLVA LDLE DCHL EDGQ LYAI LPAL SKCF KLTK FSFY GNQI SMLA MKDL LHHT ASLI HLRL ELYP VPQD SYDY HGTP LMQR MQQH CDEL MNLL KTFR NPGR VFFG TDRC DQCC NRYI YNKT IMCN CRRS Y |
| Gene ID: | 627009 |
| Uniprot: | Q5VT98 |
| Cellular Location: | |
| Calculated MW: | 55kDa |
| Observed MW: | 55kDa |
| Synonyms: | PRAMEF20, PRAMEF21, Gm13125 |
| Background: |
| UniProt Protein Function: | PRAMEF20: Belongs to the PRAME family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 1p36.21 Molecular Function:retinoic acid receptor binding Biological Process: negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell differentiation; positive regulation of cell proliferation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis |
| UniProt Code: | Q5VT98 |
| NCBI GenInfo Identifier: | 169234924 |
| NCBI Gene ID: | 627009 |
| NCBI Accession: | NP_001108549.1 |
| Molecular Weight: | Calculated: 55kDaObserved: 55kDa |
| NCBI Full Name: | novel preferentially expressed antigen in melanoma (Prame) protein |
| NCBI Synonym Full Names: | PRAME family member 20 |
| NCBI Official Symbol: | Pramef20 |
| NCBI Official Synonym Symbols: | Gm13125; EG627009 |
| NCBI Protein Information: | novel preferentially expressed antigen in melanoma (Prame) protein |
| UniProt Protein Name: | PRAME family member 20/21 |
| UniProt Gene Name: | PRAMEF20 |
| UniProt Entry Name: | PRA20_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Gm13125 antibody (CAB12619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using GM13125 antibody (CAB12619) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using GM13125 antibody (CAB12619) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using GM13125 antibody (CAB12619) at dilution of 1:100 (40x lens). |
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