Antibody Name:	Anti-GM130 Antibody
Antibody SKU:	CAB5344
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GM130 (NP_004477.3).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, HeLa, OVCAR-3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GM130 (NP_004477.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SKLA AAKK KLRE YQQR NSPG VPTG AKKK KKIK NGSN PETT TSGG CHSP EDTP KDNA ATLQ PSDD TVLP GGVP SPGA SLTS MAAS QNHD ADNV PNLM DETK TFSS TESL RQLS QQLN GLVC ESAT CVNG EGPA SSAN LKDL ESRY QQLA VALD SSYV TNKQ LNIT IEKL KQQN QEIT DQLE EEKK ECHQ KQGA LREQ LQVH IQTI GILV SEKA ELQT ALAH TQHA ARQK EGES EDLA SRLQ YSRR RVGE LERA LSAV STQQ KKAD RYNK ELTK ERDA LRLE L
Gene ID:	2801
Uniprot:	Q08379
Cellular Location:	Cytoplasm, Golgi apparatus, Peripheral membrane protein, cis-Golgi network membrane, cytoskeleton, spindle pole
Calculated MW:	70kDa/113kDa
Observed MW:	145kDa

Synonyms:

GOLGA2, GM130, golgin A2

Background:

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

UniProt Protein Function:	GM130: Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. Part of a larger oligomeric complex. Interacts with p115. Interacts with RAB1B that has been activated by GTP-binding. Interacts with GORASP1/GRASP65 and ZFPL1. Belongs to the GOLGA2 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 9q34.11 Cellular Component: Golgi membrane; Golgi apparatus; cis-Golgi network; ER to Golgi transport vesicle; nucleus Molecular Function:protein binding; syntaxin binding; protein kinase binding Biological Process: mitotic cell cycle; negative regulation of protein binding
NCBI Summary:	The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]
UniProt Code:	Q08379
NCBI GenInfo Identifier:	294862511
NCBI Gene ID:	2801
NCBI Accession:	Q08379.3
UniProt Secondary Accession:	Q08379,Q6GRM9, Q9BRB0, Q9NYF9,
UniProt Related Accession:	Q08379
Molecular Weight:	1002
NCBI Full Name:	Golgin subfamily A member 2
NCBI Synonym Full Names:	golgin A2
NCBI Official Symbol:	GOLGA2
NCBI Official Synonym Symbols:	GM130
NCBI Protein Information:	Golgin subfamily A member 2; golgin-95; SY11 protein; GM130 autoantigen; Golgi matrix protein GM130; 130 kDa cis-Golgi matrix protein; golgi autoantigen, golgin subfamily a, 2
UniProt Protein Name:	Golgin subfamily A member 2
UniProt Synonym Protein Names:	130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; Golgin-95
Protein Family:	Golgin subfamily
UniProt Gene Name:	GOLGA2
UniProt Entry Name:	GOGA2_HUMAN

	Western blot analysis of extracts of various cell lines, using GM130 antibody (CAB5344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Confocal immunofluorescence analysis of Hela cells using GM130 antibody (CAB5344) at dilution of 1:400. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug GM130 antibody (CAB5344). Western blot was performed from the immunoprecipitate using GM130 antibody (CAB5344) at a dilition of 1:1000.