Cell Biology Antibodies 11
Anti-GLUD1 Antibody (CAB7631)
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- SKU:
- CAB7631
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GLUD1 Antibody |
| Antibody SKU: | CAB7631 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 54-240 of human GLUD1 (NP_005262.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, 22Rv1, HeLa, HepG2, Mouse liver, Mouse kidney, Mouse brain, Rat liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 54-240 of human GLUD1 (NP_005262.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SEAV ADRE DDPN FFKM VEGF FDRG ASIV EDKL VEDL RTRE SEEQ KRNR VRGI LRII KPCN HVLS LSFP IRRD DGSW EVIE GYRA QHSQ HRTP CKGG IRYS TDVS VDEV KALA SLMT YKCA VVDV PFGG AKAG VKIN PKNY TDNE LEKI TRRF TMEL AKKG FIGP GIDV PAPD MSTG EREM SWIA DTY |
| Gene ID: | 2746 |
| Uniprot: | P00367 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 42kDa/46kDa/61kDa |
| Observed MW: | 50kDa |
| Synonyms: | GLUD1, GDH, GDH1, GLUD |
| Background: | This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. |
| UniProt Protein Function: | Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. |
| NCBI Summary: | This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. [provided by RefSeq, Jan 2016] |
| UniProt Code: | P00367 |
| NCBI GenInfo Identifier: | 974141156 |
| NCBI Gene ID: | 2746 |
| NCBI Accession: | NP_001305829.1 |
| UniProt Secondary Accession: | P00367,Q5TBU3, B3KV55, B4DGN5, |
| UniProt Related Accession: | P00367 |
| Molecular Weight: | 46,575 Da |
| NCBI Full Name: | glutamate dehydrogenase 1, mitochondrial isoform b |
| NCBI Synonym Full Names: | glutamate dehydrogenase 1 |
| NCBI Official Symbol: | GLUD1 |
| NCBI Official Synonym Symbols: | GDH; GDH1; GLUD |
| NCBI Protein Information: | glutamate dehydrogenase 1, mitochondrial |
| UniProt Protein Name: | Glutamate dehydrogenase 1, mitochondrial |
| UniProt Gene Name: | GLUD1 |
 | Western blot analysis of extracts of various cell lines, using GLUD1 antibody (CAB7631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using GLUD1 antibody (CAB7631) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using GLUD1 antibody (CAB7631) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophagus using GLUD1 antibody (CAB7631) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using GLUD1 antibody (CAB7631) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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