Cell Biology Antibodies 9
Anti-GLRX3 Antibody (CAB5892)
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- SKU:
- CAB5892
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GLRX3 Antibody |
| Antibody SKU: | CAB5892 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GLRX3 (NP_001186797.1). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | Raji, MCF-7, A-431, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GLRX3 (NP_001186797.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAG AAEA AVAA VEEV GSAG QFEE LLRL KAKS LLVV HFWA PWAP QCAQ MNEV MAEL AKEL PQVS FVKL EAEG VPEV SEKY EISS VPTF LFFK NSQK IDRL DGAH APEL TKKV QRHA SSGS FLPS ANEH LKED LNLR LKKL THAA PCML FMKG TPQE PRCG FSKQ MVEI LHKH NIQF SSFD IFSD EEVR QGLK AYSS WPTY PQLY VSGE LIGG LDII KELE ASEE LDTI CPKA PKLE ERLK VLTN KASV MLFM KGNK QEAK CGFS KQIL EILN STGV EYET FDIL EDEE VRQG LKAY SNWP TYPQ LYVK GELV GGLD IVKE LKEN GELL PILR GEN |
| Gene ID: | 10539 |
| Uniprot: | O76003 |
| Cellular Location: | Cytoplasm, Z line, cell cortex, myofibril, sarcomere |
| Calculated MW: | 37kDa |
| Observed MW: | 37kDa |
| Synonyms: | GLRX3, GLRX4, GRX3, GRX4, PICOT, TXNL2, TXNL3 |
| Background: | This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene. |
| UniProt Protein Function: | GLRX3: Critical negative regulator of cardiac hypertrophy and a positive inotropic regulator. May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity. |
| UniProt Protein Details: | Protein type:Oxidoreductase Chromosomal Location of Human Ortholog: 10q26 Cellular Component: cell cortex; Z disc Molecular Function:protein binding; electron carrier activity; protein kinase C binding; metal ion binding; iron-sulfur cluster binding; protein disulfide oxidoreductase activity Biological Process: cell redox homeostasis; regulation of the force of heart contraction |
| NCBI Summary: | This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010] |
| UniProt Code: | O76003 |
| NCBI GenInfo Identifier: | 37087933 |
| NCBI Gene ID: | 10539 |
| NCBI Accession: | O76003.2 |
| UniProt Related Accession: | O76003 |
| Molecular Weight: | Observed: 37 kDaPredicted: 38 kDa |
| NCBI Full Name: | Glutaredoxin-3 |
| NCBI Synonym Full Names: | glutaredoxin 3 |
| NCBI Official Symbol: | GLRX3 |
| NCBI Official Synonym Symbols: | GRX3; GRX4; GLRX4; PICOT; TXNL2; TXNL3 |
| NCBI Protein Information: | glutaredoxin-3 |
| UniProt Protein Name: | Glutaredoxin-3 |
| UniProt Synonym Protein Names: | PKC-interacting cousin of thioredoxin; PICOT; PKC-theta-interacting protein; PKCq-interacting protein; Thioredoxin-like protein 2 |
| Protein Family: | Glutaredoxin |
| UniProt Gene Name: | GLRX3 |
| UniProt Entry Name: | GLRX3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GLRX3 antibody (CAB5892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug GLRX3 antibody (CAB5892). Western blot was performed from the immunoprecipitate using GLRX3 antibody (CAB5892) at a dilition of 1:1000. |
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