Name: Anti-GLRX2 Antibody (CAB6603)
Brand: Cell Biology Antibodies 10
SKU: CAB6603
Availability: OutOfStock

Antibody Name:	Anti-GLRX2 Antibody
Antibody SKU:	CAB6603
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human GLRX2 (NP_001230328.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human GLRX2 (NP_001230328.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MESN TSSS LENL ATAP VNQI QETI SDNC VVIF SKTS CSYC TMAK KLFH DMNV NYKV VELD LLEY GNQF QDAL YKMT GERT VPRI FVNG TFIG GATD THRL HKEG KLLP LVHQ CYLK KSKR KEFQ
Gene ID:	51022
Uniprot:	Q9NS18
Cellular Location:	Mitochondrion, Nucleus
Calculated MW:	18kDa
Observed MW:	18kDa

Synonyms:

GLRX2, CGI-133, GRX2

Background:

The protein encoded by this gene is a member of the glutaredoxin family of proteins, which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms, one of which is ubiquitously expressed and localizes to mitochondria, where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms, which have more restrictive expression patterns, show cytosolic and nuclear localization, and are thought to function in cellular differentiation and transformation, possibly with a role in tumor progression.

UniProt Protein Function:	GLRX2: Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release. Belongs to the glutaredoxin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oxidoreductase; Mitochondrial Chromosomal Location of Human Ortholog: 1q31.2 Cellular Component: nucleoplasm; mitochondrion; intracellular membrane-bound organelle; nucleus Molecular Function:2 iron, 2 sulfur cluster binding; electron carrier activity; metal ion binding; protein disulfide oxidoreductase activity; protein disulfide isomerase activity; arsenate reductase (glutaredoxin) activity; glutathione disulfide oxidoreductase activity Biological Process: response to organic substance; response to temperature stimulus; DNA protection; glutathione metabolic process; response to hydrogen peroxide; regulation of transcription, DNA-dependent; protein folding; apoptosis; cell redox homeostasis; regulation of signal transduction; response to redox state; cell differentiation
NCBI Summary:	The protein encoded by this gene is a member of the glutaredoxin family of proteins, which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms, one of which is ubiquitously expressed and localizes to mitochondria, where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms, which have more restrictive expression patterns, show cytosolic and nuclear localization, and are thought to function in cellular differentiation and transformation, possibly with a role in tumor progression. [provided by RefSeq, Aug 2011]
UniProt Code:	Q9NS18
NCBI GenInfo Identifier:	73919686
NCBI Gene ID:	51022
NCBI Accession:	Q9NS18.1
UniProt Secondary Accession:	Q9NS18,Q3LR69, Q7L1N7, Q96JC0, Q9Y3D4,
UniProt Related Accession:	Q9NS18
Molecular Weight:	18,726 Da
NCBI Full Name:	Glutaredoxin-2, mitochondrial
NCBI Synonym Full Names:	glutaredoxin 2
NCBI Official Symbol:	GLRX2
NCBI Official Synonym Symbols:	GRX2; CGI-133
NCBI Protein Information:	glutaredoxin 2; bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2)
UniProt Protein Name:	Glutaredoxin-2, mitochondrial
UniProt Gene Name:	GLRX2
UniProt Entry Name:	GLRX2_HUMAN

Western blot analysis of extracts of MCF-7 cells, using GLRX2 Antibody (CAB6603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

GLRX2 Antibody (PACO09487)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)