Name: Anti-GLRA1 Antibody (CAB2958)
Brand: Signal Transduction Antibodies 2
SKU: CAB2958
Availability: OutOfStock

Antibody Name:	Anti-GLRA1 Antibody
Antibody SKU:	CAB2958
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-247 of human GLRA1 (NP_000162.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:1000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, U-251MG, Mouse testis, Mouse brain, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-247 of human GLRA1 (NP_000162.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ARSA PKPM SPSD FLDK LMGR TSGY DARI RPNF KGPP VNVS CNIF INSF GSIA ETTM DYRV NIFL RQQW NDPR LAYN EYPD DSLD LDPS MLDS IWKP DLFF ANEK GAHF HEIT TDNK LLRI SRNG NVLY SIRI TLTL ACPM DLKN FPMD VQTC IMQL ESFG YTMN DLIF EWQE QGAV QVAD GLTL PQFI LKEE KDLR YCTK HYNT GKFT CIEA RFHL ERQ
Gene ID:	2741
Uniprot:	P23415
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	51kDa/52kDa
Observed MW:	53kDa

Synonyms:	GLRA1, HKPX1, STHE
Background:	The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found.

UniProt Protein Function:	GLRA1: The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing). Defects in GLRA1 are the cause of hyperekplexia, hereditary, type 1 (HKPX1). A neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA1 sub- subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Transporter, ion channel; Transporter Chromosomal Location of Human Ortholog: 5q32 Cellular Component: postsynaptic membrane; intracellular membrane-bound organelle; integral to plasma membrane; integral to membrane; plasma membrane; cell junction; external side of plasma membrane Molecular Function:protein binding; transmitter-gated ion channel activity; extracellular-glycine-gated chloride channel activity; glycine binding; taurine binding Biological Process: regulation of action potential; startle response; synaptic transmission, glycinergic; righting reflex; acrosome reaction; chloride transport; neurological control of breathing; adult walking behavior; regulation of inhibitory postsynaptic membrane potential; neuromuscular process controlling posture; regulation of membrane potential; visual perception; negative regulation of transmission of nerve impulse; muscle contraction; neuropeptide signaling pathway; ion transport; transmembrane transport Disease: Hyperekplexia, Hereditary 1
NCBI Summary:	The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found. [provided by RefSeq, Dec 2015]
UniProt Code:	P23415
NCBI GenInfo Identifier:	116242495
NCBI Gene ID:	2741
NCBI Accession:	P23415.2
UniProt Related Accession:	P23415
Molecular Weight:	53kDa
NCBI Full Name:	Glycine receptor subunit alpha-1
NCBI Synonym Full Names:	glycine receptor alpha 1
NCBI Official Symbol:	GLRA1
NCBI Official Synonym Symbols:	STHE; HKPX1
NCBI Protein Information:	glycine receptor subunit alpha-1
UniProt Protein Name:	Glycine receptor subunit alpha-1
UniProt Synonym Protein Names:	Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit
Protein Family:	Glycine receptor
UniProt Gene Name:	GLRA1
UniProt Entry Name:	GLRA1_HUMAN

Western blot analysis of extracts of various cell lines, using GLRA1 antibody (CAB2958) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 50s.

Antibodies

GLRA1 Antibody (PACO09485)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)