Epigenetics & Nuclear Signaling Antibodies 1

Anti-GLIS3 Antibody (CAB12166)

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SKU:
CAB12166
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-GLIS3 Antibody
Antibody SKU:CAB12166
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 606-775 of human GLIS3 (NP_689842.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, BxPC-3, HepG2, A-549, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 606-775 of human GLIS3 (NP_689842.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DAGA ERFA PSAP SPHH ISPR RVPA PSSI LQRT QPPY TQQP SGSH LKSY QPET NSSF QPNG IHVH GFYG QLQK FCPP HYPD SQRI VPPV SSCS VVPS FEDC LVPT SMGQ ASFD VFHR AFST HSGI TVYD LPSS SSSL FGES LRSG AEDA TFLQ ISTV DRCP SQLS SVYT EG
Gene ID:169792
Uniprot:Q8NEA6
Cellular Location:Nucleus
Calculated MW:83kDa/99kDa
Observed MW:72kDa
Synonyms:GLIS3, NDH, ZNF515
Background:This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined.
UniProt Protein Function:GLIS3: Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'. Defects in GLIS3 are a cause of diabetes mellitus neonatal with congenital hypothyroidism (NDH). NDH is a neonatal diabetes syndrome associated with congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 9p24.2

Cellular Component: nucleus

Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Disease: Diabetes Mellitus, Neonatal, With Congenital Hypothyroidism

NCBI Summary:This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q8NEA6
NCBI GenInfo Identifier:322510143
NCBI Gene ID:169792
NCBI Accession:Q8NEA6.5
UniProt Secondary Accession:Q8NEA6,Q1PHK5, B1AL19,
UniProt Related Accession:Q8NEA6
Molecular Weight:84kDa
NCBI Full Name:Zinc finger protein GLIS3
NCBI Synonym Full Names:GLIS family zinc finger 3
NCBI Official Symbol:GLIS3  
NCBI Official Synonym Symbols:NDH; ZNF515  
NCBI Protein Information:zinc finger protein GLIS3
UniProt Protein Name:Zinc finger protein GLIS3
UniProt Synonym Protein Names:GLI-similar 3; Zinc finger protein 515
Protein Family:Zinc finger protein
UniProt Gene Name:GLIS3  
Anti-GLIS3 Antibody (CAB12166)Western blot analysis of extracts of various cell lines, using GLIS3 antibody (CAB12166) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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