Developmental Biology
Anti-GLI1 Antibody (CAB8387)
- SKU:
- CAB8387
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-GLI1 Antibody |
| Antibody SKU: | CAB8387 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human GLI1 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse testis |
| Immunogen: | Recombinant protein of human GLI1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 2735 |
| Uniprot: | P08151 |
| Cellular Location: | Cytoplasm, Nucleus, Nucleus |
| Calculated MW: | 104kDa/113kDa/117kDa |
| Observed MW: | 160KDa |
| Synonyms: | GLI1, GLI |
| Background: | This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | GLI1: Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. Interacts with KIF7. Interacts with ZIC1; the interaction enhances transcription activation. Amplified in glioblastoma cells. Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; Oncoprotein; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 12q13.2-q13.3 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; metal ion binding; microtubule binding; chromatin binding Biological Process: transcription from RNA polymerase II promoter; smoothened signaling pathway; ventral midline development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; positive regulation of transcription, DNA-dependent; digestive tract morphogenesis; notochord regression; osteoblast differentiation; cerebellar cortex morphogenesis; dorsal/ventral pattern formation; pituitary gland development; positive regulation of cell proliferation; positive regulation of smoothened signaling pathway; epidermal cell differentiation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA replication; proximal/distal pattern formation; regulation of smoothened signaling pathway; lung development |
| NCBI Summary: | This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| UniProt Code: | P08151 |
| NCBI GenInfo Identifier: | 121323 |
| NCBI Gene ID: | 2735 |
| NCBI Accession: | P08151.1 |
| UniProt Related Accession: | P08151 |
| Molecular Weight: | |
| NCBI Full Name: | Zinc finger protein GLI1 |
| NCBI Synonym Full Names: | GLI family zinc finger 1 |
| NCBI Official Symbol: | GLI1 |
| NCBI Official Synonym Symbols: | GLI; PPD1; PAPA8 |
| NCBI Protein Information: | zinc finger protein GLI1 |
| UniProt Protein Name: | Zinc finger protein GLI1 |
| UniProt Synonym Protein Names: | Glioma-associated oncogene; Oncogene GLI |
| Protein Family: | Zinc finger protein |
| UniProt Gene Name: | GLI1 |
| UniProt Entry Name: | GLI1_HUMAN |
 | Western blot analysis of extracts of Mouse testis, using GLI1 Rabbit pAb (CAB8387) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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