Name: Anti-GLE1 Antibody (CAB13207)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB13207
Availability: OutOfStock

Antibody Name:	Anti-GLE1 Antibody
Antibody SKU:	CAB13207
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GLE1 (NP_001003722.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GLE1 (NP_001003722.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPSE GRCW ETLK ALRS SDKG RLCY YRDW LLRR EDVL EECM SLPK LSSY SGWV VEHV LPHM QENQ PLSE TSPS STSA SALD QPSF VPKS PDAS SAFS PASP ATPN GTKG KDES QHTE SMVL QSSR GIKV EGCV RMYE LVHR MKGT EGLR LWQE EQER KVQA LSEM ASEQ LKRF DEWK ELKQ HKEF QDLR EVME KSSR EALG HQEK LKAE HRHR AKIL NLKL REAE QQRV KQAE QERL RKEE GQIR LRAL YALQ EEML QLSQ QLDA SEQH KALL KVDL AAFQ TRGN QLCS LISG IIRA SSES
Gene ID:	2733
Uniprot:	Q53GS7
Cellular Location:	Cytoplasm, Cytoplasm, Nucleus, nuclear pore complex
Calculated MW:	75kDa/79kDa
Observed MW:	80kDa

Synonyms:

GLE1, GLE1L, LCCS, LCCS1, hGLE1

Background:

This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GLE1: Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. May be involved in the terminal step of the mRNA transport through the nuclear pore complex (NPC). Defects in GLE1 are the cause of lethal congenital contracture syndrome type 1 (LCCS1); also known as multiple contracture syndrome type Finnish. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, micrognatia, pulmonary hypoplasia, pterygia, multiple joint contractures, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS1 leads to prenatal death. Defects in GLE1 are the cause of lethal arthrogryposis with anterior horn cell disease (LAAHD). LAAHD is characterized by fetal akinesia, arthrogryposis and motor neuron loss. LAADH fetus often survive delivery, but die early as a result of respiratory failure. Neuropathological findings resemble those of LCCS1, but are less severe. Belongs to the GLE1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Karyopherin; Nuclear export Chromosomal Location of Human Ortholog: 9q34.11 Cellular Component: extracellular space; membrane; cytoplasm; plasma membrane; nuclear pore Molecular Function:identical protein binding; protein binding Biological Process: poly(A)+ mRNA export from nucleus; mRNA export from nucleus; protein transport Disease: Lethal Arthrogryposis With Anterior Horn Cell Disease; Lethal Congenital Contracture Syndrome 1
UniProt Code:	Q53GS7
NCBI GenInfo Identifier:	51317384
NCBI Gene ID:	2733
NCBI Accession:	NP_001003722.1
UniProt Secondary Accession:	Q53GS7,O75458, Q53GT9, Q5VVU1, Q8NCP6, Q9UFL6,
UniProt Related Accession:	Q53GS7
Molecular Weight:	79,836 Da
NCBI Full Name:	nucleoporin GLE1 isoform 1
NCBI Synonym Full Names:	GLE1 RNA export mediator
NCBI Official Symbol:	GLE1
NCBI Official Synonym Symbols:	LCCS; GLE1L; LCCS1; hGLE1
NCBI Protein Information:	nucleoporin GLE1; GLE1-like protein; GLE1-like, RNA export mediator; GLE1 RNA export mediator homolog
UniProt Protein Name:	Nucleoporin GLE1
UniProt Synonym Protein Names:	GLE1-like protein
Protein Family:	Protein
UniProt Gene Name:	GLE1
UniProt Entry Name:	GLE1_HUMAN

Western blot analysis of extracts of Jurkat cells, using GLE1 antibody (CAB13207) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

Anti-GLE1 Antibody (CAB13206)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)