Name: Anti-GK Antibody (CAB6377)
Brand: Metabolism Antibodies 2
SKU: CAB6377
Availability: OutOfStock

Antibody Name:	Anti-GK Antibody
Antibody SKU:	CAB6377
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 251-530 of human GK (NP_976325.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	DU145, U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 251-530 of human GK (NP_976325.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AGAL EGVP ISGC LGDQ SAAL VGQM CFQI GQAK NTYG TGCF LLCN TGHK CVFS DHGL LTTV AYKL GRDK PVYY ALEG SVAI AGAV IRWL RDNL GIIK TSEE IEKL AKEV GTSY GCYF VPAF SGLY APYW EPSA RGII CGLT QFTN KCHI AFAA LEAV CFQT REIL DAMN RDCG IPLS HLQV DGGM TSNK ILMQ LQAD ILYI PVVK PSMP ETTA LGAA MAAG AAEG VGVW SLEP EDLS AVTM ERFE PQIN AEES EIRY STWK KAVM KSMG WVTT QSPE SGIP
Gene ID:	2710
Uniprot:	P32189
Cellular Location:	Cytoplasm, Cytoplasmic side, Mitochondrion outer membrane, Peripheral membrane protein
Calculated MW:	57kDa/58kDa/60kDa/61kDa
Observed MW:	61kDa

Synonyms:

GK, GK1, GKD

Background:

The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GLPK: Key enzyme in the regulation of glycerol uptake and metabolism. Defects in GK are the cause of GK deficiency (GKD). This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only. Belongs to the FGGY kinase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.7.1.30; Lipid Metabolism - glycerolipid; Mitochondrial; Kinase, other Chromosomal Location of Human Ortholog: Xp21.3 Cellular Component: mitochondrial outer membrane; cytosol; nucleus Molecular Function:glycerol kinase activity; protein binding; ATP binding Biological Process: triacylglycerol metabolic process; glycerol metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; glucose homeostasis; phosphorylation; glycerol-3-phosphate biosynthetic process; glycerol catabolic process Disease: Glycerol Kinase Deficiency
NCBI Summary:	The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
UniProt Code:	P32189
NCBI GenInfo Identifier:	205830913
NCBI Gene ID:	2710
NCBI Accession:	P32189.3
UniProt Secondary Accession:	P32189,Q6IQ27, Q8IVR5, Q9UMP0, Q9UMP1, A6NJP5, B2R833
UniProt Related Accession:	P32189
Molecular Weight:	58,141 Da
NCBI Full Name:	Glycerol kinase
NCBI Synonym Full Names:	glycerol kinase
NCBI Official Symbol:	GK
NCBI Official Synonym Symbols:	GK1; GKD
NCBI Protein Information:	glycerol kinase; ATP:glycerol 3-phosphotransferase; glycerokinase
UniProt Protein Name:	Glycerol kinase
UniProt Synonym Protein Names:	ATP:glycerol 3-phosphotransferase
Protein Family:	Glycerol kinase
UniProt Gene Name:	GK
UniProt Entry Name:	GLPK_HUMAN

Western blot analysis of extracts of various cell lines, using GK antibody (CAB6377) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Antibodies

GK Antibody (PACO00855)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)