Signal Transduction Antibodies 2

Anti-GIT2 Antibody (CAB15368)

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SKU:
CAB15368
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-GIT2 Antibody
Antibody SKU:CAB15368
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 450-510 of human GIT2 (NP_476511.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:A-431, LO2, Jurkat, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 450-510 of human GIT2 (NP_476511.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ASRL EKQN STPE SDYD NTPN DMEP DGMG SSRK GRQR SMVW PGDG LVPD TAEP HVAP SPTL P
Gene ID:9815
Uniprot:Q14161
Cellular Location:
Calculated MW:52kDa, 70-84kDa
Observed MW:84kDa
Synonyms:GIT2, CAT-2, CAT2, PKL
Background:This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.
UniProt Protein Function:GIT2: possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Interacts with G protein-coupled receptor kinases. Associates with paxillin. Also interacts with PIX exchange factors. Regulates Golgi and actin cytoskeletal organization. Important in integrin-mediated cell adhesion. Ten alternatively spliced isoforms have been described and additional isoforms seem to exist. Contains 1 Arf-GAP domain and 3 ANK repeats.
UniProt Protein Details:

Protein type:GAPs; GAPs, ARF; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: focal adhesion; nucleoplasm

Molecular Function:protein binding

Biological Process: regulation of G-protein coupled receptor protein signaling pathway

NCBI Summary:This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008]
UniProt Code:Q14161
NCBI GenInfo Identifier:17376322
NCBI Gene ID:9815
NCBI Accession:Q14161.2
UniProt Secondary Accession:Q14161,Q86U59, Q96CI2, Q9BV91, Q9Y5V2,
UniProt Related Accession:Q14161
Molecular Weight:70,183 Da
NCBI Full Name:ARF GTPase-activating protein GIT2
NCBI Synonym Full Names:GIT ArfGAP 2
NCBI Official Symbol:GIT2  
NCBI Official Synonym Symbols:PKL; CAT2; CAT-2  
NCBI Protein Information:ARF GTPase-activating protein GIT2
UniProt Protein Name:ARF GTPase-activating protein GIT2
UniProt Synonym Protein Names:Cool-interacting tyrosine-phosphorylated protein 2; CAT-2; CAT2; G protein-coupled receptor kinase-interactor 2; GRK-interacting protein 2
Protein Family:ARF GTPase-activating protein
UniProt Gene Name:GIT2  
UniProt Entry Name:GIT2_HUMAN
Anti-GIT2 Antibody (CAB15368)Western blot analysis of extracts of various cell lines, using GIT2 antibody (CAB15368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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