Signal Transduction Antibodies 3
Anti-GIF Antibody (CAB6914)
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- SKU:
- CAB6914
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-GIF Antibody |
| Antibody SKU: | CAB6914 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GIF (NP_005133.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse pancreas, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GIF (NP_005133.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TQTQ SSCS VPSA QEPL VNGI QVLM ENSV TSSA YPNP SILI AMNL AGAY NLKA QKLL TYQL MSSD NNDL TIGQ LGLT IMAL TSSC RDPG DKVS ILQR QMEN WAPS SPNA EASA FYGP SLAI LALC QKNS EATL PIAV RFAK TLLA NSSP FNVD TGAM ATLA LTCM YNKI PVGS EEGY RSLF GQVL KDIV EKIS MKIK DNGI IGDI YSTG LAMQ ALSV TPEP SKKE WNCK KTTD MILN EIKQ GKFH NPMS IAQI LPSL KGKT YLDV PQVT CSPD HEVQ PTLP S |
| Gene ID: | 2694 |
| Uniprot: | P27352 |
| Cellular Location: | Secreted |
| Calculated MW: | 42kDa/45kDa |
| Observed MW: | 45kDa |
| Synonyms: | GIF, IF, IFMH, INF, TCN3 |
| Background: | This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. |
| UniProt Protein Function: | GIF: Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis. Defects in GIF are the cause of hereditary intrinsic factor deficiency (IFD); also known as congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11q13 Cellular Component: extracellular space; lysosomal lumen; microvillus; apical plasma membrane; extracellular region; endosome Molecular Function:cobalamin binding Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process Disease: Intrinsic Factor Deficiency |
| NCBI Summary: | This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P27352 |
| NCBI GenInfo Identifier: | 62906845 |
| NCBI Gene ID: | 2694 |
| NCBI Accession: | P27352.2 |
| UniProt Related Accession: | P27352 |
| Molecular Weight: | |
| NCBI Full Name: | Cobalamin binding intrinsic factor |
| NCBI Synonym Full Names: | cobalamin binding intrinsic factor |
| NCBI Official Symbol: | CBLIF |
| NCBI Official Synonym Symbols: | IF; GIF; INF; IFMH; TCN3 |
| NCBI Protein Information: | cobalamin binding intrinsic factor |
| UniProt Protein Name: | Gastric intrinsic factor |
| UniProt Synonym Protein Names: | Intrinsic factor; IF; INF |
| Protein Family: | Gastric intrinsic factor |
| UniProt Gene Name: | GIF |
| UniProt Entry Name: | IF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GIF antibody (CAB6914) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of A549 cells using GIF antibody (CAB6914). Blue: DAPI for nuclear staining. |
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