Antibody Name:	Anti-GHRL Antibody
Antibody SKU:	CAB12581
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GHRL (NP_057446.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GHRL (NP_057446.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPSP GTVC SLLL LGML WLDL AMAG SSFL SPEH QRVQ QRKE SKKP PAKL QPRA LAGW LRPE DGGQ AEGA EDEL EVRF NAPF DVGI KLSG VQYQ QHSQ ALGK FLQD ILWE EAKE APAD K
Gene ID:	51738
Uniprot:	Q9UBU3
Cellular Location:	Secreted
Calculated MW:	7kDa/9kDa/11kDa/12kDa
Observed MW:	15kDa

Synonyms:

GHRL, MTLRP

Background:

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression.

UniProt Protein Function:	ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide; Cell development/differentiation; Cell cycle regulation; Apoptosis Chromosomal Location of Human Ortholog: 3p26-p25 Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region Molecular Function:growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; decidualization; positive regulation of multicellular organism growth; positive regulation of adrenocorticotropic hormone secretion; negative regulation of insulin secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; positive regulation of insulin secretion; glucose metabolic process; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; negative regulation of inflammatory response; actin polymerization and/or depolymerization; cartilage development; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis Disease: Obesity
NCBI Summary:	This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq, Oct 2008]
UniProt Code:	Q9UBU3
NCBI GenInfo Identifier:	17865471
NCBI Gene ID:	51738
NCBI Accession:	Q9UBU3.1
UniProt Secondary Accession:	Q9UBU3,Q86YP8, Q8TAT9, Q9H3R3, A8CF34, A8CF38, A8CF42 A8DN29, A8DN30,
UniProt Related Accession:	Q9UBU3
Molecular Weight:	9,972 Da
NCBI Full Name:	Appetite-regulating hormone
NCBI Synonym Full Names:	ghrelin/obestatin prepropeptide
NCBI Official Symbol:	GHRL
NCBI Official Synonym Symbols:	MTLRP
NCBI Protein Information:	appetite-regulating hormone; motilin-related peptide; growth hormone secretagogue; ghrelin/obestatin preprohormone; growth hormone-releasing peptide; prepro-appetite regulatory hormone; ghrelin, growth hormone secretagogue receptor ligand
UniProt Protein Name:	Appetite-regulating hormone
UniProt Synonym Protein Names:	Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46Cleaved into the following 3 chains:Ghrelin-27; Ghrelin-28; Ghrelin; Obestatin
Protein Family:	Ancylostoma secreted protein
UniProt Gene Name:	GHRL
UniProt Entry Name:	GHRL_HUMAN

	Western blot analysis of extracts of rat liver, using GHRL antibody (CAB12581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using GHRL antibody (CAB12581) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human rectum using GHRL antibody (CAB12581) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human rectal cancer using GHRL antibody (CAB12581) at dilution of 1:100 (40x lens).