Metabolism Antibodies 2
Anti-GGT1 Antibody (CAB1776)
- SKU:
- CAB1776
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-GGT1 Antibody |
| Antibody SKU: | CAB1776 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 370-569 of human GGT1 (NP_001027537.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 370-569 of human GGT1 (NP_001027537.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KPEF YTPD DGGT AHLS VVAE DGSA VSAT STIN LYFG SKVR SPVS GILF NNEM DDFS SPSI TNEF GVPP SPAN FIQP GKQP LSSM CPTI MVGQ DGQV RMVV GAAG GTQI TTAT ALAI IYNL WFGY DVKR AVEE PRLH NQLL PNVT TVER NIDQ AVTA ALET RHHH TQIA STFI AVVQ AIVR TAGG WAAA SDSR KGGE PAGY |
| Gene ID: | 2678 |
| Uniprot: | P19440 |
| Cellular Location: | Cell membrane, Single-pass type II membrane protein |
| Calculated MW: | 24kDa/39kDa/61kDa |
| Observed MW: | 60-70kDa |
| Synonyms: | GGT1, CD224, D22S672, D22S732, GGT, GGT 1, GTG |
| Background: | The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. |
| UniProt Protein Function: | GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - taurine and hypotaurine; Membrane protein, integral; Other Amino Acids Metabolism - glutathione; EC 3.4.19.13; EC 3.4.19.14; Transferase; Other Amino Acids Metabolism - cyanoamino acid; Other Amino Acids Metabolism - selenoamino acid; Lipid Metabolism - arachidonic acid; EC 2.3.2.2 Chromosomal Location of Human Ortholog: 22q11.23 Cellular Component: anchored to external side of plasma membrane; extracellular space; integral to membrane; plasma membrane Molecular Function:protein binding; gamma-glutamyltransferase activity Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; regulation of inflammatory response; glutathione catabolic process; glutathione biosynthetic process; arachidonic acid metabolic process; zymogen activation; spermatogenesis Disease: Glutathionuria |
| NCBI Summary: | The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014] |
| UniProt Code: | P19440 |
| NCBI GenInfo Identifier: | 93140064 |
| NCBI Gene ID: | 2678 |
| NCBI Accession: | P19440.2 |
| UniProt Secondary Accession: | P19440,Q08247, Q14404, Q8TBS1, Q9UMK1, |
| UniProt Related Accession: | P19440 |
| Molecular Weight: | 569 |
| NCBI Full Name: | Gamma-glutamyltranspeptidase 1 |
| NCBI Synonym Full Names: | gamma-glutamyltransferase 1 |
| NCBI Official Symbol: | GGT1 |
| NCBI Official Synonym Symbols: | GGT; GTG; CD224; GGT 1; D22S672; D22S732 |
| NCBI Protein Information: | gamma-glutamyltranspeptidase 1; glutathione hydrolase 1; leukotriene-C4 hydrolase |
| UniProt Protein Name: | Gamma-glutamyltranspeptidase 1 |
| UniProt Synonym Protein Names: | Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain |
| Protein Family: | Gamma-glutamyltranspeptidase |
| UniProt Gene Name: | GGT1 |
| UniProt Entry Name: | GGT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GGT1 antibody (CAB1776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using GGT1 antibody (CAB1776) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using GGT1 antibody (CAB1776) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using GGT1 antibody (CAB1776) at dilution of 1:100 (40x lens). |
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