Cell Biology Antibodies 9

Anti-GGH Antibody (CAB5464)

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SKU:
CAB5464
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GGH Antibody
Antibody SKU:CAB5464
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-230 of human GGH (NP_003869.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:HeLa, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-230 of human GGH (NP_003869.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RPHG DTAK KPII GILM QKCR NKVM KNYG RYYI AASY VKYL ESAG ARVV PVRL DLTE KDYE ILFK SING ILFP GGSV DLRR SDYA KVAK IFYN LSIQ SFDD GDYF PVWG TCLG FEEL SLLI SGEC LLTA TDTV DVAM PLNF TGGQ LHSR MFQN FPTE LLLS LAVE PLTA NFHK WSLS VKNF TMNE KLKK FFNV LTTN TDGK IEFI ST
Gene ID:8836
Uniprot:Q92820
Cellular Location:Lysosome, Melanosome, Secreted, extracellular space
Calculated MW:35kDa
Observed MW:32kDa
Synonyms:GGH, GH
Background:This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate.
UniProt Protein Function:GGH: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha- glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Belongs to the peptidase C26 family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Cofactor and Vitamin Metabolism - folate biosynthesis; Secreted; Protease; EC 3.4.19.9

Chromosomal Location of Human Ortholog: 8q12.3

Cellular Component: extracellular space; nucleus; vacuole

Molecular Function:exopeptidase activity; omega peptidase activity

Biological Process: tetrahydrofolylpolyglutamate metabolic process

NCBI Summary:This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008]
UniProt Code:Q92820
NCBI GenInfo Identifier:6016127
NCBI Gene ID:8836
NCBI Accession:Q92820.2
UniProt Related Accession:Q92820
Molecular Weight:35,964 Da
NCBI Full Name:Gamma-glutamyl hydrolase
NCBI Synonym Full Names:gamma-glutamyl hydrolase
NCBI Official Symbol:GGH  
NCBI Official Synonym Symbols:GH  
NCBI Protein Information:gamma-glutamyl hydrolase
UniProt Protein Name:Gamma-glutamyl hydrolase
UniProt Synonym Protein Names:Conjugase; GH; Gamma-Glu-X carboxypeptidase
Protein Family:Gamma-glutamyl hydrolase
UniProt Gene Name:GGH  
UniProt Entry Name:GGH_HUMAN
Anti-GGH Antibody (CAB5464)Western blot analysis of extracts of various cell lines, using GGH antibody (CAB5464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-GGH Antibody (CAB5464)Immunofluorescence analysis of A-549 cells using GGH antibody (CAB5464).
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