Cell Biology Antibodies 13
Anti-GGA1 Antibody (CAB17126)
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- SKU:
- CAB17126
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-GGA1 Antibody (CAB17126) |
| Antibody SKU: | CAB17126 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GGA1 (NP_001001560.1). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GGA1 (NP_001001560.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEPA MEPE TLEA RINR ATNP LNKE LDWA SING FCEQ LNED FEGP PLAT RLLA HKIQ SPQE WEAI QALT VLET CMKS CGKR FHDE VGKF RFLN ELIK VVSP |
| Cellular Location: | Endosome membrane, Golgi apparatus, Peripheral membrane protein, trans-Golgi network membrane |
| Calculated MW: | 9kDa/61kDa/62kDa/66kDa/70kDa/72kDa |
| Observed MW: | 70KDa |
| Synonyms: | GGA1 |
| Background: | This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
 | Western blot analysis of extracts of Mouse brain, using GGA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
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