Cell Biology Antibodies 13

Anti-GGA1 Antibody (CAB17126)

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SKU:
CAB17126
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-GGA1 Antibody (CAB17126)
Antibody SKU:CAB17126
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GGA1 (NP_001001560.1).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GGA1 (NP_001001560.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEPA MEPE TLEA RINR ATNP LNKE LDWA SING FCEQ LNED FEGP PLAT RLLA HKIQ SPQE WEAI QALT VLET CMKS CGKR FHDE VGKF RFLN ELIK VVSP
Cellular Location:Endosome membrane, Golgi apparatus, Peripheral membrane protein, trans-Golgi network membrane
Calculated MW:9kDa/61kDa/62kDa/66kDa/70kDa/72kDa
Observed MW:70KDa
Synonyms:GGA1
Background:This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Western blot analysis of extracts of Mouse brain, using GGA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Western blot analysis of extracts of Mouse brain, using GGA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
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