Name: Anti-GFRA2 Antibody (CAB2954)
Brand: Cell Biology Antibodies 8
SKU: CAB2954
Availability: OutOfStock

Antibody Name:	Anti-GFRA2 Antibody
Antibody SKU:	CAB2954
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 360-464 of human GFRA2 (NP_001486.4).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, LO2, HepG2, HeLa, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 360-464 of human GFRA2 (NP_001486.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DVNV SPKG PSFQ ATQA PRVE KTPS LPDD LSDS TSLG TSVI TTCT SVQE QGLK ANNS KELS MCFT ELTT NIIP GSNK VIKP NSGP SRAR PSAA LTVL SVLM LKLA L
Gene ID:	2675
Uniprot:	O00451
Cellular Location:	Cell membrane, GPI-anchor, Lipid-anchor
Calculated MW:	36kDa/39kDa/51kDa
Observed MW:	34kDa/55kDa

Synonyms:

GFRA2, GDNFRB, NRTNR-ALPHA, NTNRA, RETL2, TRNR2

Background:

Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GFRA2: Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, GPI anchor Chromosomal Location of Human Ortholog: 8p21.3 Cellular Component: extrinsic to membrane; plasma membrane Molecular Function:glial cell line-derived neurotrophic factor receptor activity Biological Process: nervous system development; negative regulation of protein amino acid autophosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway
NCBI Summary:	Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Code:	O00451
NCBI GenInfo Identifier:	118582303
NCBI Gene ID:	2675
NCBI Accession:	O00451.2
UniProt Related Accession:	O00451
Molecular Weight:
NCBI Full Name:	GDNF family receptor alpha-2
NCBI Synonym Full Names:	GDNF family receptor alpha 2
NCBI Official Symbol:	GFRA2
NCBI Official Synonym Symbols:	NTNRA; RETL2; TRNR2; GDNFRB; NRTNR-ALPHA
NCBI Protein Information:	GDNF family receptor alpha-2
UniProt Protein Name:	GDNF family receptor alpha-2
UniProt Synonym Protein Names:	GDNF receptor beta; GDNFR-beta; Neurturin receptor alpha; NRTNR-alpha; NTNR-alpha; RET ligand 2; TGF-beta-related neurotrophic factor receptor 2
Protein Family:	GDNF family receptor
UniProt Gene Name:	GFRA2
UniProt Entry Name:	GFRA2_HUMAN

Western blot analysis of extracts of various cell lines, using GFRA2 antibody (CAB2954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

GFRA2 Antibody (PACO09456)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)