Cell Biology Antibodies 6
Anti-GFPT2 Antibody (CAB15374)
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- SKU:
- CAB15374
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GFPT2 Antibody |
| Antibody SKU: | CAB15374 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-260 of human GFPT2 (NP_005101.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HeLa, A375, NIH/3T3, Mouse heart, Mouse pancreas, Mouse testis, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-260 of human GFPT2 (NP_005101.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RGSP LLIG VRSK YKLS TEQI PILY RTCT LENV KNIC KTRM KRLD SSAC LHAV GDKA VEFF F |
| Gene ID: | 9945 |
| Uniprot: | O94808 |
| Cellular Location: | |
| Calculated MW: | 76kDa |
| Observed MW: | 77kDa |
| Synonyms: | GFPT2, GFAT, GFAT 2, GFAT2 |
| Background: |
| UniProt Protein Function: | GFAT2: Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Highest levels of expression in heart, placenta, and spinal cord. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.16; Transferase; Carbohydrate Metabolism - amino sugar and nucleotide sugar Chromosomal Location of Human Ortholog: 5q34-q35 Cellular Component: cytosol Molecular Function:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; protein binding Biological Process: energy reserve metabolic process; fructose 6-phosphate metabolic process; UDP-N-acetylglucosamine biosynthetic process; UDP-N-acetylglucosamine metabolic process |
| UniProt Code: | O94808 |
| NCBI GenInfo Identifier: | 6226820 |
| NCBI Gene ID: | 9945 |
| NCBI Accession: | O94808.3 |
| UniProt Secondary Accession: | O94808,Q53XM2, Q9BWS4, |
| UniProt Related Accession: | O94808 |
| Molecular Weight: | 76,931 Da |
| NCBI Full Name: | Glutamine--fructose-6-phosphate aminotransferase |
| NCBI Synonym Full Names: | glutamine-fructose-6-phosphate transaminase 2 |
| NCBI Official Symbol: | GFPT2 |
| NCBI Official Synonym Symbols: | GFAT; GFAT2; GFAT 2 |
| NCBI Protein Information: | glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 |
| UniProt Protein Name: | Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 |
| UniProt Synonym Protein Names: | D-fructose-6-phosphate amidotransferase 2; Glutamine:fructose-6-phosphate amidotransferase 2; GFAT 2; GFAT2; Hexosephosphate aminotransferase 2 |
| Protein Family: | Glutamine--fructose-6-phosphate aminotransferase |
| UniProt Gene Name: | GFPT2 |
| UniProt Entry Name: | GFPT2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GFPT2 antibody (CAB15374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunofluorescence analysis of L929 cells using GFPT2 antibody (CAB15374) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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