Anti-GFPT1 Antibody (CAB20873)
- SKU:
- CAB20873
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-GFPT1 Antibody (CAB20873) |
| Antibody SKU: | CAB20873 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry, Immunoprecipitation |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human GFPT1. |
| Application: | Western blotting, Immunohistochemistry, Immunoprecipitation |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, RD, Mouse large intestine, Rat testis, Rat spleen, Rat lung |
| Immunogen: | Recombinant protein of Human GFPT1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | |
| Calculated MW: | 76kDa/78kDa |
| Observed MW: | 80kDa |
| Synonyms: | GFPT1, CMS12, CMSTA1, GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1L, MSLG |
| Background: | This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. |
 | Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug GFPT1 antibody . Western blot was performed from the immunoprecipitate using GFPT1 antibody at a dilition of 1:1000. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using GFPT1 Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using GFPT1 Rabbit mAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using GFPT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
 | Western blot analysis of extracts of various cell lines, using GFPT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
 | Western blot analysis of extracts of Mouse large intestine, using GFPT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
Related Products
Human GFPT1 ELISA Kit (HUFI04144)
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीकी
Human GFPT1 Recombinant Protein (RPPB1686)
Enzymes Recombinant Proteins
system_update_altDatasheetsystem_update_altMSDS
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक