Metabolism Antibodies 1
Anti-GFM2 Antibody (CAB10158)
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- SKU:
- CAB10158
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-GFM2 Antibody |
| Antibody SKU: | CAB10158 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 520-779 of human GFM2 (NP_115756.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:4000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, U-87MG, LO2, A-431, HT-1080, K-562, Mouse liver, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 520-779 of human GFM2 (NP_115756.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KVRL DPDS GQTV LCGM GELH IEII HDRI KREY GLET YLGP LQVA YRET ILNS VRAT DTLD RTLG DKRH LVTV EVEA RPIE TSSV MPVI EFEY AESI NEGL LKVS QEAI ENGI HSAC LQGP LLGS PIQD VAIT LHSL TIHP GTST TMIS ACVS RCVQ KALK KADK QVLE PLMN LEVT VARD YLSP VLAD LAQR RGNI QEIQ TRQD NKVV IGFV PLAE IMGY STVL RTLT SGSA TFAL ELST YQAM NPQD QNTL LNRR SGLT |
| Gene ID: | 84340 |
| Uniprot: | Q969S9 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 57kDa/67kDa/81kDa/86kDa |
| Observed MW: | 86kDa |
| Synonyms: | GFM2, EF-G2mt, EFG2, MRRF2, MST027, MSTP027, RRF2, RRF2mt, hEFG2, mEF-G 2, mEF-G2 |
| Background: | Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors, which is a GTPase that plays a role at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA . Its role in the regulation of normal mitochondrial function and in disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | GFM2: Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation. Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 5q13.3 Cellular Component: intracellular; mitochondrial matrix Molecular Function:GTPase activity; protein binding; translation elongation factor activity Biological Process: mitochondrial translation; ribosome disassembly |
| NCBI Summary: | Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors, which is a GTPase that plays a role at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA . Its role in the regulation of normal mitochondrial function and in disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q969S9 |
| NCBI GenInfo Identifier: | 526479814 |
| NCBI Gene ID: | 84340 |
| NCBI Accession: | NP_001268231.1 |
| UniProt Secondary Accession: | Q969S9,Q8N6D8, Q8WYI0, Q9H6Z1, A0AR28, |
| UniProt Related Accession: | Q969S9 |
| Molecular Weight: | 26.6kDa |
| NCBI Full Name: | ribosome-releasing factor 2, mitochondrial isoform 4 |
| NCBI Synonym Full Names: | G elongation factor mitochondrial 2 |
| NCBI Official Symbol: | GFM2 |
| NCBI Official Synonym Symbols: | EFG2; RRF2; MRRF2; hEFG2; MST027; RRF2mt; EF-G2mt; MSTP027; mEF-G 2 |
| NCBI Protein Information: | ribosome-releasing factor 2, mitochondrial |
| UniProt Protein Name: | Ribosome-releasing factor 2, mitochondrial |
| UniProt Synonym Protein Names: | Elongation factor G 2, mitochondrial |
| UniProt Gene Name: | GFM2 |
 | Western blot analysis of extracts of various cell lines, using GFM2 antibody (CAB10158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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