Cell Biology Antibodies 1
Anti-GFAP Antibody (CAB0237)
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- SKU:
- CAB0237
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GFAP Antibody |
| Antibody SKU: | CAB0237 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RGTN ESLE RQMR EQEE RHVR EAAS YQEA LARL EEEG QSLK DEMA RHLQ EYQD LLNV KLAL DIEI ATYR KLLE GEEN RITI PVQT FSNL QIRE TSLD TKSV SEGH LKRN IVVK TVEM RDGE VIKE SKQE HKDV M |
| Gene ID: | 2670 |
| Uniprot: | P14136 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 49kDa/50kDa |
| Observed MW: | 50KDa |
| Synonyms: | ALXDRD, GFAP |
| Background: | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 17q21 Cellular Component: membrane; cytoplasm; intermediate filament; cytosol Molecular Function:integrin binding; structural constituent of cytoskeleton; kinase binding Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; intermediate filament organization; neurite regeneration; astrocyte development Disease: Alexander Disease |
| NCBI Summary: | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P14136 |
| NCBI GenInfo Identifier: | 121135 |
| NCBI Gene ID: | 2670 |
| NCBI Accession: | P14136.1 |
| UniProt Secondary Accession: | P14136,Q53H98, Q5D055, Q6ZQS3, Q7Z5J6, Q7Z5J7, Q96KS4 Q96P18, Q9UFD0, B2RD44, D3DX59, E9PAX3, |
| UniProt Related Accession: | P14136 |
| Molecular Weight: | 432 |
| NCBI Full Name: | Glial fibrillary acidic protein |
| NCBI Synonym Full Names: | glial fibrillary acidic protein |
| NCBI Official Symbol: | GFAP |
| NCBI Protein Information: | glial fibrillary acidic protein |
| UniProt Protein Name: | Glial fibrillary acidic protein |
| Protein Family: | Glial fibrillary acidic protein |
| UniProt Gene Name: | GFAP |
| UniProt Entry Name: | GFAP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GFAP Rabbit pAb (CAB0237) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat brain using GFAP Rabbit pAb (CAB0237) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human brain using GFAP Rabbit pAb (CAB0237) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-251 MG cells using GFAP Rabbit pAb (CAB0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of rat brain using GFAP Rabbit pAb (CAB0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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