Signal Transduction Antibodies 2
Anti-GET4 Antibody (CAB16190)
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- SKU:
- CAB16190
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-GET4 Antibody |
| Antibody SKU: | CAB16190 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human GET4 (NP_057033.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, A-549, HeLa, 293T, Mouse brain, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human GET4 (NP_057033.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA AAMA EQES ARNG GRNR GGVQ RVEG KLRA SVEK GDYY EAHQ MYRT LFFR YMSQ SKHT EARE LMYS GALL FFSH GQQN SAAD LSML VLES LEKA EVEV ADEL LENL AKVF SLMD PNSP ERVT FVSR ALKW SSGG SGKL GHPR LHQL LALT LWKE QNYC ESRY HFLH SADG EGCA NMLV EYST SRGF RSEV DMFV AQAV LQFL CLKN KSSA SVVF TTYT QKHP SIED GPPF VEPL LNFI WFLL LAVD GGKL TVFT VLCE QYQP SLRR DPMY NEYL DRIG QLFF GVPP KQTS SYGG LLGN LLTS LMGS SEQE DGEE SPSD GSPI ELD |
| Gene ID: | 51608 |
| Uniprot: | Q7L5D6 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 30kDa/36kDa |
| Observed MW: | 36kDa |
| Synonyms: | GET4, C7orf20, CEE, CGI-20, TRC35 |
| Background: |
| UniProt Protein Function: | GET4: Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting. Belongs to the GET4 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 7p22.3 Cellular Component: cytosol Biological Process: transport |
| UniProt Code: | Q7L5D6 |
| NCBI GenInfo Identifier: | 38570062 |
| NCBI Gene ID: | 51608 |
| NCBI Accession: | NP_057033.2 |
| UniProt Related Accession: | Q7L5D6 |
| Molecular Weight: | 30kDa |
| NCBI Full Name: | Golgi to ER traffic protein 4 homolog |
| NCBI Synonym Full Names: | golgi to ER traffic protein 4 |
| NCBI Official Symbol: | GET4 |
| NCBI Official Synonym Symbols: | CEE; TRC35; CGI-20; C7orf20 |
| NCBI Protein Information: | Golgi to ER traffic protein 4 homolog |
| UniProt Protein Name: | Golgi to ER traffic protein 4 homolog |
| UniProt Synonym Protein Names: | Conserved edge-expressed protein; Transmembrane domain recognition complex 35 kDa subunit; TRC35 |
| Protein Family: | Golgi to ER traffic protein |
| UniProt Gene Name: | GET4 |
| UniProt Entry Name: | GET4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GET4 antibody (CAB16190) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
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