Antibody Name:	Anti-Gephyrin Antibody
Antibody SKU:	CAB8572
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 520-769 of human GPHN (NP_065857.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Mouse brain, Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 520-769 of human GPHN (NP_065857.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TVGV TEVE VNKF PVVA VMST GNEL LNPE DDLL PGKI RDSN RSTL LATI QEHG YPTI NLGI VGDN PDDL LNAL NEGI SRAD VIIT SGGV SMGE KDYL KQVL DIDL HAQI HFGR VFMK PGLP TTFA TLDI DGVR KIIF ALPG NPVS AVVT CNLF VVPA LRKM QGIL DPRP TIIK ARLS CDVK LDPR PEYH RCIL TWHH QEPL PWAQ STGN QMSS RLMS MRSA NGLL MLPP KTEQ YVEL HKGE VVDV MVIG RL
Gene ID:	10243
Uniprot:	Q9NQX3
Cellular Location:	Cell junction, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton, postsynaptic cell membrane, synapse
Calculated MW:	79kDa/83kDa
Observed MW:	103kDa

Synonyms:

GPHN, GEPH, GPH, GPHRYN, HKPX1, MOCODC, gephyrin

Background:

This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known.

UniProt Protein Function:	gephyrin: Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. Homotrimer. Interacts with GABARAP. Inhibited by copper and tungsten. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.7.7.75; Adaptor/scaffold; EC 2.10.1.1 Chromosomal Location of Human Ortholog: 14q23.3 Cellular Component: cytoplasm; dendrite; plasma membrane; postsynaptic membrane Molecular Function:protein binding Biological Process: Mo-molybdopterin cofactor biosynthetic process; molybdenum incorporation into molybdenum-molybdopterin complex; molybdopterin cofactor biosynthetic process Disease: Hyperekplexia, Hereditary 1; Molybdenum Cofactor Deficiency, Complementation Group C
NCBI Summary:	This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NQX3
NCBI GenInfo Identifier:	13431554
NCBI Gene ID:	10243
NCBI Accession:	Q9NQX3.1
UniProt Secondary Accession:	Q9NQX3,Q96KU4, Q9H4E9, Q9P2G2,
UniProt Related Accession:	Q9NQX3
Molecular Weight:	83,448 Da
NCBI Full Name:	Gephyrin
NCBI Synonym Full Names:	gephyrin
NCBI Official Symbol:	GPHN
NCBI Official Synonym Symbols:	GPH; GEPH; HKPX1; GPHRYN; MOCODC
NCBI Protein Information:	gephyrin
UniProt Protein Name:	Gephyrin
UniProt Synonym Protein Names:	Domain E
Protein Family:	Gephyrin
UniProt Gene Name:	GPHN
UniProt Entry Name:	GEPH_HUMAN

	Western blot analysis of extracts of various cell lines, using GPHN antibody (CAB8572) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse lung using GPHN antibody (CAB8572) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of rat brain using GPHN antibody (CAB8572) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.