Epigenetics & Nuclear Signaling Antibodies 3

Anti-GEMIN2 Antibody (CAB3082)

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SKU:
CAB3082
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-GEMIN2 Antibody
Antibody SKU:CAB3082
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human GEMIN2
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat
Immunogen:A synthetic peptide of human GEMIN2
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8487
Uniprot:O14893
Cellular Location:Cytoplasm, Nucleus, gem
Calculated MW:4kDa/28kDa/29kDa/31kDa
Observed MW:35kDa
Synonyms:GEMIN2, SIP1, SIP1-delta
Background:This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.
UniProt Protein Function:SIP1: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. Belongs to the gemin-2 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA splicing

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nucleolus; SMN complex; cytosol; nucleus

Molecular Function:protein binding

Biological Process: RNA splicing, via transesterification reactions; spliceosomal snRNP biogenesis; RNA splicing; spliceosome assembly; gene expression; mRNA processing

NCBI Summary:This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. [provided by RefSeq, Aug 2011]
UniProt Code:O14893
NCBI GenInfo Identifier:6094289
NCBI Gene ID:8487
NCBI Accession:O14893.1
UniProt Related Accession:O14893
Molecular Weight:31585
NCBI Full Name:Gem-associated protein 2
NCBI Synonym Full Names:gem nuclear organelle associated protein 2
NCBI Official Symbol:GEMIN2  
NCBI Official Synonym Symbols:SIP1; SIP1-delta  
NCBI Protein Information:gem-associated protein 2
UniProt Protein Name:Gem-associated protein 2
UniProt Synonym Protein Names:Component of gems 2; Survival of motor neuron protein-interacting protein 1; SMN-interacting protein 1
Protein Family:SNF1 protein kinase
UniProt Gene Name:GEMIN2  
UniProt Entry Name:GEMI2_HUMAN
Anti-GEMIN2 Antibody (CAB3082)Western blot analysis of extracts of Jurkat cells, using GEMIN2 antibody (CAB3082) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-GEMIN2 Antibody (CAB3082)Immunohistochemistry of paraffin-embedded rat liver using GEMIN2 Antibody (CAB3082) at dilution of 1:200 (40x lens).
Anti-GEMIN2 Antibody (CAB3082)Immunohistochemistry of paraffin-embedded human lung cancer using GEMIN2 Antibody (CAB3082) at dilution of 1:100 (40x lens).
Anti-GEMIN2 Antibody (CAB3082)Immunohistochemistry of paraffin-embedded mouse testis using GEMIN2 Antibody (CAB3082) at dilution of 1:100 (40x lens).
Anti-GEMIN2 Antibody (CAB3082)Immunofluorescence analysis of A549 cells using GEMIN2 antibody (CAB3082). Blue: DAPI for nuclear staining.
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